Metabolic changes associated with shoot formation in tobacco callus cultures

Technical Report · Sun Aug 01 04:00:00 EDT 1982

DOI:https://doi.org/10.2172/5602732· OSTI ID:5602732

Grady, K L

Callus tissue derived from Nicotiana tabacum L. stem pith parenchyma cells was grown either on medium which maintains the callus in an undifferentiated state, or on medium which induces the formation of shoots. Two complementary types of studies were performed with the goal of establishing metabolic markers for the initiation of shoot formation: one designed to characterize the flow of radioactive sucrose into various metabolic pools, and one which allowed measurement of intermediary metabolite concentrations. In the former, callus tissue was incubated in (U-/sup 14/C)sucrose for periods up to one hour, and patterns of metabolite labelling in tissue grown on shoot-forming and non-shoot-forming media were compared. In the latter studies, tissue was grown for an entire subculture period on non-shoot-forming medium labelled with (U-/sup 14/C)sucrose, then subcultured to labelled non-shoot-forming or shoot-forming media, and sampled at intervals during the first week of growth. 189 references.

• Research Organization: Lawrence Berkeley Lab., CA (USA)
• DOE Contract Number: AC03-76SF00098
• OSTI ID: 5602732
• Report Number(s): LBL-15180; ON: DE83017678
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BIOSYNTHESIS
CARBOHYDRATES
CARBON 14 COMPOUNDS
DATA
DISACCHARIDES
EXPERIMENTAL DATA
GROWTH
IN VITRO
INFORMATION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
METABOLISM
METABOLITES
NICOTIANA
NUMERICAL DATA
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PLANT GROWTH
PLANT STEMS
PLANTS
SACCHARIDES
SACCHAROSE
SYNTHESIS
TISSUE CULTURES
TRACER TECHNIQUES