Thymine lesions produced by ionizing radiation in double-stranded DNA

Journal Article · Tue Jul 16 04:00:00 EDT 1985 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00336a032· OSTI ID:6255698

Breimer, L H; Lindahl, T

A DNA glycosylase which catalyzes the release of thymine residues damaged by ring saturation, fragmentation, or ring contraction from double-stranded DNA has been used to characterize such base derivatives in gamma-irradiated DNA. It is shown by chromatographic analysis that irradiation of DNA in neutral solution generates the ring-saturated forms cis-thymine glycol, trans-thymine glycol, and a monohydroxydihydrothymine, probably 6-hydroxy-5,6-dihydrothymine. The latter compound is only observed after irradiation under hypoxic conditions. The ring-contracted thymine derivative 5-hydroxy-5-methylhydantoin is also formed, and it is the major lesion after irradiation of DNA under O2. Ring-fragmented products such as methyltartronylurea were only generated in small quantities. Isolation and analysis of the DNA from gamma-irradiated human cells also revealed the formation of ring-saturated thymine derivatives, but 5-hydroxy-5-methylhydantoin was not found in this case.

• Research Organization: Imperial Cancer Research Fund, London, England
• OSTI ID: 6255698
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 15; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AZINES
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CONSTITUENTS
DNA
ELECTROMAGNETIC RADIATION
ENZYMES
ESCHERICHIA COLI
GAMMA RADIATION
GLYCOSYL HYDROLASES
HETEROCYCLIC COMPOUNDS
HYDROLASES
HYDROXY COMPOUNDS
IONIZING RADIATIONS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PLASMIDS
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
THYMINE
URACILS