Thymine fragment damage retained in the DNA polynucleotide chain after gamma irradiation in aerated solutions. II
Journal Article
·
· Radiat. Res.; (United States)
The chemical modifications of the thymine fragment due to gamma irradiation of aerated solutions of DNA, which remain linked to the polynucleotide chain, were determined by hydrolysis. The formic acid hydrolysis was carried out at 90/sup 0/C and in this way cis- and trans-5,6-dihydroxy-5,6-dihydrothymine (1 and 2), 5-hydroxy-5-methylhydantoin (3), formylurea (4), and urea (5) were released from the DNA chain backbone by rupture of the N-glycosidic bond. These defects were characterized unambiguously by chromatography and microreactions. These hydrolysis conditions made it possible to measure the quantities of the main defects 1,2, (sum) and 3 which were borne by the polynucleotide chain. By taking advantage of the fact that carbonic oxide and pyruvamide were released directly from the chain during the irradiation the formation of the N-formamidodeoxyribose DNA defect (6) was demonstrated. A mechanism to explain the formation of these defects, 1 to 5 and 6, pyruvamide and carbonic oxide, is suggested.
- Research Organization:
- Centre d'Etudes Nucleaires de Grenoble, France
- OSTI ID:
- 5144227
- Journal Information:
- Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 72:2; ISSN RAREA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AZINES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CHEMICAL REACTIONS
CHROMATOGRAPHY
DECOMPOSITION
DISPERSIONS
DNA
ELECTROMAGNETIC RADIATION
GAMMA RADIATION
HETEROCYCLIC COMPOUNDS
HYDROLYSIS
HYDROXY COMPOUNDS
IONIZING RADIATIONS
KINETICS
LYSIS
MIXTURES
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
REACTION KINETICS
SEPARATION PROCESSES
SOLUTIONS
SOLVOLYSIS
THYMINE
URACILS
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AZINES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CHEMICAL REACTIONS
CHROMATOGRAPHY
DECOMPOSITION
DISPERSIONS
DNA
ELECTROMAGNETIC RADIATION
GAMMA RADIATION
HETEROCYCLIC COMPOUNDS
HYDROLYSIS
HYDROXY COMPOUNDS
IONIZING RADIATIONS
KINETICS
LYSIS
MIXTURES
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
REACTION KINETICS
SEPARATION PROCESSES
SOLUTIONS
SOLVOLYSIS
THYMINE
URACILS