Postreceptor defects causing insulin resistance in normoinsulinemic non-insulin-dependent diabetes mellitus

Journal Article · Fri Oct 01 00:00:00 EDT 1982 · Diabetes; (United States)

DOI:https://doi.org/10.2337/diab.31.10.911· OSTI ID:6255063

Bolinder, J; Ostman, J; Arner, P

The mechanisms of the diminished hypoglycemic response to insulin in non-insulin-dependent diabetes mellitus (NIDDM) with normal levels of circulating plasma insulin were investigated. Specific binding of mono-/sup 125/I (Tyr A14)-insulin to isolated adipocytes and effects of insulin (5--10,000 microunits/ml) on glucose oxidation and lipolysis were determined simultaneously in subcutaneous adipose tissue of seven healthy subjects of normal weight and seven untreated NIDDM patients with normal plasma insulin levels. The two groups were matched for age, sex, and body weight. Insulin binding, measured in terms of receptor number and affinity, was normal in NIDDM, the total number of receptors averaging 350,000 per cell. Neither sensitivity nor the maximum antilipolytic effect of insulin was altered in NIDDM patients as compared with control subjects; the insulin concentration producing half the maximum effect (ED50) was 10 microunits/ml. As regards the effect of insulin on glucose oxidation, for the control subjects ED50 was 30 microunits/ml, whereas in NIDDM patients, insulin exerted no stimulatory effect. The results obtained suggest that the effect of insulin on glucose utilization in normoinsulinemic NIDDM may be diminished in spite of normal insulin binding to receptors. The resistance may be due solely to postreceptor defects, and does not involve antilipolysis.

• Research Organization: Department of Medicine and the Research Center, Huddinge Hospital, Huddinge, Sweden
• OSTI ID: 6255063
• Journal Information: Diabetes; (United States), Vol. 31:10
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLUCOSE
METABOLISM
INSULIN
CHEMICAL BONDS
RECEPTORS
ADIPOSE TISSUE
BIOLOGICAL FUNCTIONS
COMPARATIVE EVALUATIONS
DIABETES MELLITUS
IODINE 125
LABELLED COMPOUNDS
OXIDATION
PATIENTS
QUANTITY RATIO
ALDEHYDES
ANIMAL TISSUES
BETA DECAY RADIOISOTOPES
BODY
CARBOHYDRATES
CHEMICAL REACTIONS
CONNECTIVE TISSUE
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ENDOCRINE DISEASES
FUNCTIONS
HEXOSES
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
METABOLIC DISEASES
MONOSACCHARIDES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HORMONES
RADIOISOTOPES
SACCHARIDES
TISSUES
551001* - Physiological Systems- Tracer Techniques
550501 - Metabolism- Tracer Techniques