Comparative DNA damage and repair induced by misonidazole, CB 1954 and RSU 1069

Journal Article · Sat Apr 01 04:00:00 EST 1989 · Int. J. Radiat. Oncol., Biol. Phys.; (United States)

DOI:https://doi.org/10.1016/0360-3016(89)90902-4· OSTI ID:6253261

Dale, L D; Widdick, D A; Edwards, D I; Biol, G I

We have studied the ability of CB 1954, misonidazole, and RSU 1069 to induce biologically relevant DNA damage in single- and double-stranded phi X174 DNA under oxic, anoxic, and anoxic reductive conditions using a double transfection technique. In addition, the ability of the three drugs to induce the SOS repair response in E. coli under the same conditions was measured. Whereas the relative order of DNA damage was RSU 1069 greater than CB 1954 greater than misonidazole the order in inducing SOS repair was RSU 1069 greater than misonidazole greater than CB 1954. Drug-induced damage by RSU 1069 involves enhanced damage by endonuclease III suggesting drug-induced pyrimidine damage. There appears to be no correlation between drug-induced damage and the degree of SOS repair induction. Thus it appears that enzymes other than, or in addition to, those of the SOS repair system are involved in the repair of DNA damage induced by these drugs.

• Research Organization: North East London Polytechnic (England)
• OSTI ID: 6253261
• Journal Information: Int. J. Radiat. Oncol., Biol. Phys.; (United States), Journal Name: Int. J. Radiat. Oncol., Biol. Phys.; (United States) Vol. 16:4; ISSN IOBPD
• Country of Publication: United States
• Language: English

Related Subjects

560130* -- Radiation Effects on Microorganisms
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANOXIA
ANTINEOPLASTIC DRUGS
AZOLES
BACTERIA
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
COMPARATIVE EVALUATIONS
DNA
DNA REPAIR
DRUGS
ESCHERICHIA COLI
HETEROCYCLIC COMPOUNDS
IMIDAZOLES
MICROORGANISMS
MISONIDAZOLE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
RADIOSENSITIZERS
RECOVERY
REPAIR