Nippostrongylus brasiliensis: radioresistant IgE antibody-forming cells in infected rats
In Nippostrongylus brasiliensis-infected rats, anti-N. brasiliensis IgE antibody production was observed at 20 weeks postinfection, long after the worms, as a source of antigen, had been expelled. The persistent IgE production was not abrogated after whole body irradiation (800 R) administered at 12 or 20 weeks, suggesting the participation of radioresistant IgE-forming cells. Help of T cells and recruitment of B memory cells in the irradiated rats seems to be ruled out by the findings that the irradiation completely inhibited the initiation of anti-N. brasiliensis IgE production in rats shortly after the infection with N. brasiliensis or after primary and secondary immunization with N. brasiliensis-antigen. Moreover, clearance of anti-N. brasiliensis IgE antibody from circulation did not seem to be crucially affected by the irradiation. The radioresistant cells forming anti-N. brasiliensis IgE were most productive in mesenteric lymph nodes as compared to other lymph nodes. The recognition of antigens fractionated by chromatography on Sephadex G-200 was the same for IgE-forming cells from rats 12 weeks after infection as for those from 3 weeks after infection. Based on these results, one of the mechanisms of persistent elevation of IgE antibody in the host infected with helminth parasites might be explained by the participation of radioresistant IgE-forming cells.
- Research Organization:
- Jikei Univ. School of Medicine, Tokyo (Japan)
- OSTI ID:
- 6252778
- Journal Information:
- Exp. Parasitol.; (United States), Vol. 68:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
IMMUNOGLOBULINS
BIOSYNTHESIS
LYMPHOCYTES
RADIOSENSITIVITY
NEMATODES
VIABILITY
ANTIBODIES
ANTIBODY FORMATION
ANTIGENS
BLOOD-PLASMA CLEARANCE
CHROMATOGRAPHY
LYMPH NODES
PARASITIC DISEASES
RATS
WHOLE-BODY IRRADIATION
ANIMAL CELLS
ANIMALS
ASCHELMINTHES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CLEARANCE
CONNECTIVE TISSUE CELLS
DISEASES
EXTERNAL IRRADIATION
GLOBULINS
HELMINTHS
INFECTIOUS DISEASES
IRRADIATION
LEUKOCYTES
LYMPHATIC SYSTEM
MAMMALS
MATERIALS
ORGANIC COMPOUNDS
PROTEINS
RODENTS
SEPARATION PROCESSES
SOMATIC CELLS
SYNTHESIS
VERTEBRATES
560152* - Radiation Effects on Animals- Animals