Intestinal mucosal mast cells from rats infected with Nippostrongylus brasiliensis contain protease-resistant chondroitin sulfate di-B proteoglycans
Rats infected with the helminth Nippostrongylus brasiliensis were injected i.p. with 2 mCi of (/sup 35/S) sulfate on days 13, 15, 17, and 19 after infection. The intestines were removed from animals on day 20 or 21 after infection, the intestinal cells were obtained by collagenase treatment and mechanical dispersion of the tissue, and the /sup 35/S-labeled mucosal mast cells (MMC) were enriched to 60 to 65% purity by Percoll centrifugation. The isolated proteoglycans were of approx. 150,000 m.w., were resistant to pronase degradation, and contained highly sulfated chondroitin sulfate side chains. The presence in normal mammalian cells of chondroitin sulfate proteoglycans that contain a high percentage of the unusual disulfated di-B disaccharide has not been previously reported. The rat intestinal MMC proteoglycans are the first chondroitin sulfate proteoglycans that have been isolated from an enriched populations of normal mast cells. They are homologous to the chondroitin sulfate-rich proteoglycans of the transformed rat basophilic leumekia-1 cell and the cultured interleukin 3-dependent mouse bone marrow-derived mast cell, in that these chondroitin sulfate proteoglycans are all highly sulfated, protease-resistant proteoglycans.
- Research Organization:
- Harvard Medical School, Boston, MA
- OSTI ID:
- 5322945
- Journal Information:
- J. Immunol.; (United States), Vol. 137:1
- Country of Publication:
- United States
- Language:
- English
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GLUCOPROTEINS
BIOCHEMICAL REACTION KINETICS
HELMINTHS
INFECTIVITY
MAST CELLS
MOLECULAR BIOLOGY
ANIMAL CELLS
CHONDROITIN
INTESTINES
MUCOUS MEMBRANES
SULFATES
SULFUR 35
TRACER TECHNIQUES
AMINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOHYDRATES
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
EVEN-ODD NUCLEI
GASTROINTESTINAL TRACT
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
MEMBRANES
MUCOPOLYSACCHARIDES
NUCLEI
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
POLYSACCHARIDES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
SACCHARIDES
SOMATIC CELLS
SULFUR COMPOUNDS
SULFUR ISOTOPES
550201* - Biochemistry- Tracer Techniques
550301 - Cytology- Tracer Techniques