Beta/sub 1/-adrenoceptors in rat hepatoma, desensitization by isoproterenol and phorbol-myristate-acetate
Journal Article
·
· Life Sci.; (United States)
The beta-adrenergic responsiveness of hepatocytes obtained from hypothyroid rats and of a transplantable hepatoma cell line (AS-30D) were studied by measuring the accumulation of cyclic AMP. The potency order for agonists in hepatocytes was: isoproterenol > epinephrine >> norepinephrine whereas in the hepatoma cells the potency order was: isoproterenol > norepinephrine /equivalent to/ epinephrine. The effect of isoproterenol was antagonized in hepatocytes by low concentrations of ICI 118551 and only partially by concentrations of atenolol as high as 100 ..mu..M. In hepatome cells the effect of isoproterenol was inhibited by both antagonists with the potency order atenolol > ICI 118551. These data indicate that in hepatocytes the effect is mediated by beta/sub 2/-adrenoceptors whereas in hepatoma cells it is through beta/sub 1/-adrenoceptors. Preincubation of hepatoma cells with isoproterenol or phorbol-myristate-acetate diminished the subsequent beta-adrenergic responsiveness of the cells. Interestingly, when both isoproterenol and phorbol-myristate-acetate were present during the preincubation the beta-adrenergic desensitization observed was bigger than that induced by any of these agents alone.
- Research Organization:
- Universidad Nacional Autonoma de Mexico
- OSTI ID:
- 6252030
- Journal Information:
- Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 44:23; ISSN LIFSA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADRENAL HORMONES
ADRENALINE
AMP
ANIMAL CELLS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL ACCUMULATION
BIOLOGICAL EFFECTS
CARCINOGENS
CARDIOTONICS
CARDIOVASCULAR AGENTS
DISEASES
DRUGS
ESTERS
HEPATOMAS
KINETICS
LIVER CELLS
MAMMALS
MEMBRANE PROTEINS
NEOPLASMS
NEUROREGULATORS
NORADRENALINE
NUCLEOTIDES
ORGANIC COMPOUNDS
PHORBOL ESTERS
PROTEINS
RATS
REACTION KINETICS
RECEPTORS
RODENTS
SOMATIC CELLS
SYMPATHOMIMETICS
SYNERGISM
VERTEBRATES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADRENAL HORMONES
ADRENALINE
AMP
ANIMAL CELLS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL ACCUMULATION
BIOLOGICAL EFFECTS
CARCINOGENS
CARDIOTONICS
CARDIOVASCULAR AGENTS
DISEASES
DRUGS
ESTERS
HEPATOMAS
KINETICS
LIVER CELLS
MAMMALS
MEMBRANE PROTEINS
NEOPLASMS
NEUROREGULATORS
NORADRENALINE
NUCLEOTIDES
ORGANIC COMPOUNDS
PHORBOL ESTERS
PROTEINS
RATS
REACTION KINETICS
RECEPTORS
RODENTS
SOMATIC CELLS
SYMPATHOMIMETICS
SYNERGISM
VERTEBRATES