Mutagenicity of diesel exhaust particle extracts: influence of fuel composition in two diesel engines

Journal Article · · Fundam. Appl. Toxicol.; (United States)

DOI:https://doi.org/10.1016/S0272-0590(82)80062-6· OSTI ID:6250803

Clark, C R ^[1]; Henderson, T R; Royer, R E; Brooks, A L; McClellan, R O; Marshall, W F; Naman, T M

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1. Lovelace Inhalation Toxicology Research Inst., Albuquerque, NM

The influence of diesel fuel composition on mutagenicity of exhaust particle associated organic compounds has been investigated using nine fuels varying in aromatic content and distillation properties. The tests were conducted with Oldsmobile Delta-88 and Peugot 504 diesel cars operated according to the EPA Federal Test Procedure. The particulate exhaust from each test was collected on a filter, extracted in dichloromethane and the resulting extract evaluated for mutagenicity in Salmonella strain TA-100. Mutagenicity of extracts of particles collected from the Oldsmobile were highest in the higher aromatic content fuels (> 30%) but similar for intermediate (20%) and low (13%) aromatic content fuels. No influence of aromaticity on mutagenicity was observed in samples collected from the Peugeot under the same conditions. Thus, fuel aromatic content may enhance the production of mutagenic combustion products at higher concentrations, but may be dependent upon engine type. A good correlation was observed between mutagenicity of the particle extracts and the initial boiling point of the fuel (r = 0.89). Gas chromatography/mass spectrometric analysis of the aromatic fraction of the fuels showed that the fuel producing the most mutagenic combustion products was highest in phenanthrene type compounds.

• OSTI ID: 6250803
• Journal Information: Fundam. Appl. Toxicol.; (United States), Journal Name: Fundam. Appl. Toxicol.; (United States) Vol. 2; ISSN FAATD
• Country of Publication: United States
• Language: English

Related Subjects

02 PETROLEUM
020600 -- Petroleum-- Health & Safety
560302* -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AROMATICS
AUTOMOBILES
BACTERIA
CHEMICAL COMPOSITION
CHROMATOGRAPHY
CONDENSED AROMATICS
DATA
DIESEL ENGINES
DIESEL FUELS
ENGINES
EXHAUST GASES
EXPERIMENTAL DATA
FLUIDS
GAS CHROMATOGRAPHY
GASEOUS WASTES
GASES
HEAT ENGINES
HYDROCARBONS
INFORMATION
INTERNAL COMBUSTION ENGINES
MASS SPECTROSCOPY
MICROORGANISMS
MUTAGEN SCREENING
MUTAGENESIS
NUMERICAL DATA
ORGANIC COMPOUNDS
PARTICLES
PARTICULATES
PETROLEUM PRODUCTS
PHENANTHRENE
SALMONELLA
SALMONELLA TYPHIMURIUM
SCREENING
SEPARATION PROCESSES
SPECTROSCOPY
VEHICLES
WASTES