The liver plays an important role in the regulation of somatostatin-14 in the rat

Journal Article · Tue Jan 01 04:00:00 EST 1991 · American Journal of Surgery; (USA)

DOI:https://doi.org/10.1016/0002-9610(91)90382-N· OSTI ID:6249081

Raper, S E; Kothary, P C; Kokudo, N; DelValle, J; Eckhauser, F E ^[1]

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1. Univ. of Michigan Medical Center, Ann Arbor (USA)

Since little is known about the in vivo disposition of circulating somatostatin-14 (SRIF-14), we examined hepatic processing of SRIF-14 in the rat. Three minutes after the intraportal injection of iodine 125 ({sup 125}I)-labeled SRIF-14, 16.0 +/- 2.0% of the injected dose is localized to the liver. In the presence of unlabeled SRIF-14, hepatic uptake can be decreased by 68%. Five minutes after the intraportal injection of {sup 125}I-SRIF-14, 9.5 +/- 1.4% of the tracer is localized to the liver, more than any other organ tested. Serial collections of bile reveal peak radioactivity at between 10 and 20 minutes. Simultaneous administration of unlabeled SRIF-14 decreases biliary radioactivity by 40%. HPLC analysis of radioactive bile reveals a chromatographic profile similar to that of intact SRIF and is 73% immunoprecipitable by an anti-SRIF antibody. Pretreatment with chloroquine, a lysosomal enzyme inhibitor, does not significantly decrease biliary radioactivity. We conclude that the data are consistent with saturable hepatic uptake and predominantly nonlysosomal transcellular transport.

• OSTI ID: 6249081
• Journal Information: American Journal of Surgery; (USA), Journal Name: American Journal of Surgery; (USA) Vol. 161:1; ISSN 0002-9610; ISSN AJSUA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
ENZYME INHIBITORS
GLANDS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIQUID COLUMN CHROMATOGRAPHY
LIVER
MAMMALS
METABOLISM
NUCLEI
ODD-EVEN NUCLEI
ORGANS
RADIOISOTOPES
RATS
RODENTS
SEPARATION PROCESSES
SOMATOSTATIN
TRACER TECHNIQUES
VERTEBRATES