Observation of a noncovalent ribonuclease S-protein/S-peptide complex by electrospray ionization mass spectrometry

Journal Article · Wed May 19 04:00:00 EDT 1993 · Journal of the American Chemical Society; (United States)

DOI:https://doi.org/10.1021/ja00063a079· OSTI ID:6247782

Loo, R R.O.; Goodlett, D R; Smith, R D; Loo, J A ^[1]

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1. Pacific Northwest Lab., Richland, WA (United States)

Electrospray ionization mass spectrometry (ESI-MS) investigations of noncovalently-bound complexes are of great interest because of their relevance to solution biochemistry. ESI-MS is particularly well suited to studies of weak interactions because it is very gentle and it allows studies to be performed under physiological or near-physiological solution conditions. However, it is not yet fully understood which weakly-bound complexes known to exist in solution will be observable by ESI-MS, or what minimum binding strength may be required for ESI-MS observation. Also unclear is whether complexes observed by ESI-MS reflect only species present in the bulk solution, or whether complexes also reflect aggregation in electrospray-generated microdroplets. We address some of these issues by employing ribonuclease S (RNase-S) as a test of the applicability of ESI-MS for analysis of noncovalent complexes. These results show that the ESI mass spectra of R Nase-S can reflect the specificity of binding in solution. 33 refs., 2 figs.

• DOE Contract Number: AC06-76RL01830
• OSTI ID: 6247782
• Journal Information: Journal of the American Chemical Society; (United States), Journal Name: Journal of the American Chemical Society; (United States) Vol. 115:10; ISSN JACSAT; ISSN 0002-7863
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102 -- Chemical & Spectral Procedures
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
BIOCHEMISTRY
CHEMISTRY
COMPLEXES
DISPERSIONS
ENZYMES
ESTERASES
HYDROLASES
MASS SPECTROSCOPY
MIXTURES
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
PROTEINS
RNA-ASE
SOLUTIONS
SPECTROSCOPY