Skip to main content
U.S. Department of Energy
Office of Scientific and Technical Information

The Observation of Chaperone-Ligand Noncovalent Complexes with Electrospray Ionization Mass Spectrometry

Journal Article · · Protein Science
FTICR-MS was applied for the study of noncovalent chaperone SecB-ligand complexes produced in solution and examined in the gas phase with the aid of electrospray ionization (ESI).
Research Organization:
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC05-76RL01830
OSTI ID:
1777111
Report Number(s):
PNNL-SA-29533
Journal Information:
Protein Science, Journal Name: Protein Science Journal Issue: 5 Vol. 7
Country of Publication:
United States
Language:
English

References (36)

Peptide binding by chaperone SecB: implications for recognition of nonnative structure journal July 1992
Electrospray mass spectrometric investigation of the chaperone SecB journal March 1996
Crystallization of the chaperone protein SecB journal August 1995
No specific recognition of leader peptide by SecB, a chaperone involved in protein export journal May 1990
Cytochrome c Folding Kinetics Studied by Time-Resolved Electrospray Ionization Mass Spectrometry journal May 1997
Mapping of the Binding Frame for the Chaperone SecB within a Natural Ligand, Galactose-binding Protein journal October 1995
Infrared Multiphoton Dissociation of Large Multiply Charged Ions for Biomolecule Sequencing journal September 1994
Selected ion accumulation of noncovalent complexes in a fourier transform ion cyclotron resonance mass spectrometer journal January 1995
Observation of the Noncovalent Quaternary Associations of Proteins by Electrospray Ionization Mass Spectrometry journal June 1994
Cytosolic factor purified from Escherichia coli is necessary and sufficient for the export of a preprotein and is a homotetramer of SecB. journal April 1989
Interaction of SecB with intermediates along the folding pathway of maltose-binding protein: Interaction of SecB with folding intermediates journal June 1995
Collisionally activated dissociation and tandem mass spectrometry of intact hemoglobin β-chain variant proteins with electrospray ionization journal February 1993
Observation of Duplex DNA-Drug Noncovalent Complexes by Electrospray Ionization Mass Spectrometry journal June 1994
Probing the oligomeric structure of an enzyme by electrospray ionization time-of-flight mass spectrometry. journal July 1996
Small volume and low flow-rate electrospray lonization mass spectrometry of aqueous samples journal November 1993
Verification of 50- to 100-mer DNA and RNA sequences with high-resolution mass spectrometry. journal March 1995
Detection of noncovalent receptor-ligand complexes by mass spectrometry journal July 1991
In-Trap Cleanup of Proteins from Electrospray Ionization Using Soft Sustained Off-Resonance Irradiation with Fourier Transform Ion Cyclotron Resonance Mass Spectrometry journal January 1998
Binding of SecB to ribosome-bound polypeptides has the same characteristics as binding to full-length, denatured proteins journal February 1997
Chaperone SecB from Escherichia coli Mediates Kinetic Partitioning via a Dynamic Equilibrium with Its Ligands journal August 1997
Characterization of PCR Products from Bacilli Using Electrospray Ionization FTICR Mass Spectrometry journal January 1996
A kinetic partitioning model of selective binding of nonnative proteins by the bacterial chaperone SecB journal January 1991
On-Line Microdialysis Sample Cleanup for Electrospray Ionization Mass Spectrometry of Nucleic Acid Samples journal January 1996
The structural basis of sequence-independent peptide binding by OppA protein journal June 1994
The crystal structures of the oligopeptide-binding protein OppA complexed with tripeptide and tetrapeptide ligands journal December 1995
Kinetic Partitioning journal November 1997
Reflecting time-of-flight mass spectrometer with an electrospray ion source and orthogonal extraction journal January 1994
Observation of large subunit protein complexes by electrospray ionization mass spectrometry journal January 1995
Electrospray ion source. Another variation on the free-jet theme journal September 1984
Determination of the binding frame of the chaperone SecB within the physiological ligand oligopeptide-binding protein journal August 1997
Dissociation of tetrameric ions of noncovalent streptavidin complexes formed by electrospray ionization journal June 1995
Micro-electrospray mass spectrometry: Ultra-high-sensitivity analysis of peptides and proteins journal July 1994
Direct observation of a DNA quadruplex by electrospray ionization mass spectrometry journal March 1993
Use of a nitrocellulose film substrate in matrix-assisted laser desorption/ionization mass spectrometry for DNA mapping and screening journal October 1995
High-resolution accurate mass measurements of biomolecules using a new electrospray ionization ion cyclotron resonance mass spectrometer journal July 1993
Electrospray ionization for mass spectrometry of large biomolecules journal October 1989

Similar Records

Observation of a noncovalent ribonuclease S-protein/S-peptide complex by electrospray ionization mass spectrometry
Journal Article · Wed May 19 00:00:00 EDT 1993 · Journal of the American Chemical Society; (United States) · OSTI ID:6247782

Acceleration of metal–ligand complexation kinetics by electrospray ionization
Journal Article · Thu Nov 02 00:00:00 EDT 2017 · Analyst · OSTI ID:1596324

Observation of the noncovalent quaternary associations of proteins by electrospray ionization mass spectrometry
Journal Article · Wed Jun 15 00:00:00 EDT 1994 · Journal of the American Chemical Society; (United States) · OSTI ID:7201175