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Title: Ethanol inhibition kinetics of Kluyveromyces marxianus grown on Jerusalem artichoke juice

Journal Article · · Appl. Environ. Microbiol.; (United States)
OSTI ID:6247405

The kinetics of ethanol inhibition on cell growth and ethanol production by Kluyveromyces marxianus UCD (FST) 55-82 were studied during batch growth. The liquid medium contained 10% (weight/volume) inulin-type sugars derived from an extract of Jerusalem artichoke (Helianthus tuberosus) tubers, supplemented with small amounts of Tween 80, oleic acid, and corn steep liquor. Initial ethanol concentrations ranging from 0 to 80 g/liter in the liquid medium were used to study the inhibitory effect of ethanol on the following parameters: maximum specific growth rate (mu max), cell and ethanol yields, and sugar utilization. It was found that as the initial ethanol concentration increased from 0 to 80 g/liter, and maximum specific growth rate of K. marxianus cells decreased from 0.42 to 0.09/hour, whereas the ethanol and cell yields and sugar utilization remained almost constant. A simple kinetic model was used to correlate the mu max results and the rates of cell and ethanol production, and the appropriate constants were evaluated. (Refs. 22).

Research Organization:
Chemical and Biochemical Engineering, Faculty of Engineering Science, The Univ of Western Ontario, London, Ontario N6A 5B9, Canada
OSTI ID:
6247405
Journal Information:
Appl. Environ. Microbiol.; (United States), Vol. 44:6
Country of Publication:
United States
Language:
English