Stimulation of proteoglycans by IGF I and II in microvessel and large vessel endothelial cells
Endothelial cells were cultured from bovine capillaries and pulmonary arteries, and the effect of insulinlike growth factor (IGF) I and II (multiplication-stimulating activity) and insulin on the synthesis of proteoglycans was determined. IGF I and II stimulated /sup 35/SO/sub 4/ incorporation into proteoglycans in a dose-dependent manner in both microvessel and pulmonary artery endothelial cells with maximum threefold increases. In pulmonary artery cells, the IGFs caused a general stimulation of all classes of glycosaminoglycan-containing proteoglycans. In microvessel endothelial cells, the IGFs appeared to preferentially increase heparan sulfate-containing proteoglycans. Insulin, at concentrations up to 10/sup -6/ M, had no effect on the synthesis of proteoglycans in either microvessel or pulmonary arterial endothelial cells. Thus, the IGFs stimulate the synthesis of proteoglycans in both microvessel and large vessel endothelial cells, a property that is not mimicked by insulin. Because vascular endothelial cells are bathed by IGFs in vivo, such IGF-mediated functions are likely to be significant in both the normal physiology of vascular endothelium and in disease states such as diabetes mellitus.
- Research Organization:
- Univ. of Iowa, Iowa City (USA)
- OSTI ID:
- 6244608
- Journal Information:
- Am. J. Physiol.; (United States), Vol. 253:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
GLUCOPROTEINS
BIOSYNTHESIS
GROWTH FACTORS
BIOLOGICAL EFFECTS
LABELLED COMPOUNDS
ARTERIES
BLOOD VESSELS
CARBON 14 COMPOUNDS
CATTLE
DOSE-RESPONSE RELATIONSHIPS
ENDOTHELIUM
INSULIN
IODINE 125
LEUCINE
PROLINE
SULFATES
SULFUR 35
TRITIUM COMPOUNDS
AMINES
AMINO ACIDS
ANIMAL TISSUES
ANIMALS
AZOLES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
GLYCOPROTEINS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
LIGHT NUCLEI
MAMMALS
MITOGENS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PEPTIDE HORMONES
PROTEINS
PYRROLES
PYRROLIDINES
RADIOISOTOPES
RUMINANTS
SACCHARIDES
SULFUR COMPOUNDS
SULFUR ISOTOPES
SYNTHESIS
TISSUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques