Molecular cloning and long terminal repeat sequences of human endogenous retrovirus genes related to types A and B retrovirus genes
Journal Article
·
· J. Virol.; (United States)
OSTI ID:6244336
By using a DNA fragment primarily encoding the reverse transcriptase (pol) region of the Syrian hamster intracisternal A particle (IAP; type A retrovirus) gene as a probe, human endogenous retrovirus genes, tentatively termed HERV-K genes, were cloned from a fetal human liver gene library. Typical HERV-K genes were 9.1 or 9.4 kilobases in length, having long terminal repeats (LTRs) of ca. 970 base pairs. Many structural features commonly observed on the retrovirus LTRs, such as the TATAA box, polyadenylation signal, and terminal inverted repeats, were present on each LTR, and a lysine (K) tRNA having a CUU anticodon was identified as a presumed primer tRNA. The HERV-K LTR, however, had little sequence homology to either the IAP LTR or other typical oncovirus LTRs. By filter hybridization, the number of HERV-K genes was estimated to be ca. 50 copies per haploid human genome. The cloned mouse mammary tumor virus (type B) gene was found to hybridize with both the HERV-K and IAP genes to essentially the same extent.
- Research Organization:
- Kitasato Univ., Kanagawa (Japan)
- OSTI ID:
- 6244336
- Journal Information:
- J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 58:3; ISSN JOVIA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Direct repeats located within a large family of human endogenous retroviral LTRs have transcriptional suppressor activity in human cells
Nucleotide sequence of a human tRNA gene heterocluster
Properties of dispersed, highly repeated DNA within and near the hamster CAD gene
Journal Article
·
Thu Sep 01 00:00:00 EDT 1994
· American Journal of Human Genetics
·
OSTI ID:133854
Nucleotide sequence of a human tRNA gene heterocluster
Conference
·
Thu May 01 00:00:00 EDT 1986
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
·
OSTI ID:5019658
Properties of dispersed, highly repeated DNA within and near the hamster CAD gene
Journal Article
·
Sun Feb 28 23:00:00 EST 1982
· Mol. Cell. Biol.; (United States)
·
OSTI ID:5608042
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ATP
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CLONING
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DNA
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
GENES
GLANDS
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
LIVER
MAMMALS
MAN
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ODD-ODD NUCLEI
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
ORGANS
PARASITES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
RADIOISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
VIRUSES
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ATP
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CLONING
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DNA
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
GENES
GLANDS
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
LIVER
MAMMALS
MAN
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ODD-ODD NUCLEI
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
ORGANS
PARASITES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
RADIOISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
VIRUSES