Properties of dispersed, highly repeated DNA within and near the hamster CAD gene

Journal Article · Mon Mar 01 04:00:00 EST 1982 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.2.3.302· OSTI ID:5608042

Padgett, R A; Wahl, G M; Stark, G R

Dispersed, highly repeated DNA sequences were found within and near the Syrian hamster gene coding for the multifunctional protein CAD. Most of the repeated sequences were homologous to each other and had similar properties. They hybridized to many cytoplasmic polyadenylated RNAs and to 7S and 4.5S cytoplasmic non-polyadenylated RNAs. Cloned DNA fragments containing repeated sequences were transcribed in vitro by RNA polymerase III. The repeated sequences from Syrian hamsters share many properties with the Alu family of repetitive DNA from humans. The hamster sequences were homologous to total repetitive human DNA but only very weakly homologous to two cloned members of the human Alu family.

• Research Organization: Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139
• OSTI ID: 5608042
• Journal Information: Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 2:3; ISSN MCEBD
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ADENYLIC ACID
ANIMALS
CELL CULTURES
CHEMICAL PROPERTIES
CHROMOSOMAL ABERRATIONS
CLONE CELLS
CLONING
CODONS
DISPERSIONS
DNA
DNA SEQUENCING
DNA-CLONING
ENZYMES
GENE REGULATION
GENES
HAMSTERS
HUMAN POPULATIONS
HYBRIDIZATION
IN VITRO
MAMMALS
MUTATIONS
NUCLEIC ACIDS
NUCLEOTIDES
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
POPULATIONS
PROTEINS
RNA POLYMERASES
RNA PROCESSING
RODENTS
STRUCTURAL CHEMICAL ANALYSIS
STRUCTURE-ACTIVITY RELATIONSHIPS
TRANSCRIPTION
TRANSFERASES
VERTEBRATES