Structure of the gene for CAD, the multifunctional protein that initiates UMP synthesis in Syrian hamster cells

Journal Article · Mon Mar 01 04:00:00 EST 1982 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.2.3.293· OSTI ID:5521163

Padgett, R A; Wahl, G M; Stark, G R

Two adjacent fragments of genomic DNA spanning the gene for CAD, which encodes the first three enzymes of UMP biosynthesis, were cloned from a mutant Syrian hamster cell line containing multiple copies of this gene. The mutant was selected for resistance to N-(phosphonacetyl)-L-aspartate, a potent and specific inhibitor of aspartate transcarbamylase, the second enzyme in the pathway. The sizes and positions of about 37 intervening sequences within the 25-kilobase CAD gene were mapped by electron microscopy, and the locations of the 5' and 3' ends of the 7.9-kilobase CAD mRNA were established by electron microscopy and by other hybridization methods. The coding sequences are small (100 to 400 bases), as are most of the intervening sequences (50 to 300 bases). However, there are also several large intervening sequences of up to 5,000 bases each. Two small cytoplasmic polyadenylated RNAs are transcribed from a region just beyond the 5' end of the CAD gene, and their abundance reflects the degree of gene amplification.

• Research Organization: Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139
• OSTI ID: 5521163
• Journal Information: Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 2:3; ISSN MCEBD
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
AMYLASE
ANIMALS
ASPARTIC ACID
BIOSYNTHESIS
CARBAMATES
CARBONIC ACID DERIVATIVES
CARBOXYLIC ACID SALTS
CARBOXYLIC ACIDS
CELL CULTURES
CHROMOSOMAL ABERRATIONS
CLONE CELLS
CLONING
DNA-CLONING
ELECTRON MICROSCOPY
ENZYME INDUCTION
ENZYMES
GENE AMPLIFICATION
GENE MUTATIONS
GENE REGULATION
GENES
GENETIC MAPPING
GENOME MUTATIONS
GLYCOSYL HYDROLASES
HAMSTERS
HYBRIDIZATION
HYDROLASES
MAMMALS
MAPPING
MESSENGER-RNA
MICROSCOPY
MUTANTS
MUTATIONS
NUCLEIC ACIDS
O-GLYCOSYL HYDROLASES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
RNA
RNA PROCESSING
RODENTS
STRUCTURE-ACTIVITY RELATIONSHIPS
SYNTHESIS
TRANSCRIPTION
TRANSFERASES
VERTEBRATES