Effect of hypoxia on thallium kinetics in cultured chick myocardial cells

Journal Article · Tue Sep 01 04:00:00 EDT 1987 · J. Nucl. Med.; (United States)

OSTI ID:6243239

Friedman, B J; Beihn, R; Friedman, J P

To assess the effect of hypoxia on cellular thallium-201 (/sup 201/Tl) uptake and washout independent of coronary flow, we studied thallium kinetics during normoxia and hypoxia in cultured chick ventricular cells. Monolayers of contracting ventricular cells grown on coverslips were placed in a chamber and perfused to asymptote with media containing /sup 201/Tl. Perfusates were equilibrated with 5% CO/sub 2/-95% air or 5% CO/sub 2/-95% nitrogen for normoxia and hypoxia, respectively. Washout thallium kinetics were then observed during perfusion with unlabeled media. Twenty paired experiments were performed, randomly alternating the sequence of normoxia and hypoxia. Pharmacokinetics for thallium were determined by computer using standard formulae. Thallium uptake and washout were best described by assuming that intracellular thallium was contained within a single compartment. Cellular thallium uptake, as well as transfer rate constants for thallium uptake and for thallium washout during normoxia and hypoxia, were compared using paired t-tests. During normoxia and hypoxia, respectively, thallium uptake was 22 +/- 7% and 19 +/- 7% of asymptote (p less than 0.01); the compartmental rate constant for uptake by the cell was 0.16 +/- 0.07 min-1 and 0.15 +/- 0.06 min-1 (N.S.); and the transfer rate constant for washout from the cell was 0.26 +/- 0.06 min-1 and 0.23 +/- 0.05 min-1 (p less than 0.01). We conclude that there was a small (14%) decrease in thallium uptake during hypoxia. The rate of thallium uptake and washout was slightly less during hypoxia, although only the rate of washout was significantly less. These data show that cellular accumulation of thallium and the rate of washout of thallium were minimally decreased by hypoxia independent of blood flow.

• Research Organization: Univ. of Kentucky Medical Center, Lexington
• OSTI ID: 6243239
• Journal Information: J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 28:9; ISSN JNMEA
• Country of Publication: United States
• Language: English

Related Subjects

560162* -- Radionuclide Effects
Kinetics
& Toxicology-- Animals
Plants
Microorganisms
& Cells
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANOXIA
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIRDS
BLOOD FLOW
BODY
CARDIOVASCULAR SYSTEM
CHICKENS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
FOWL
HEART
HEAVY NUCLEI
IN VITRO
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MUSCLES
MYOCARDIUM
NUCLEI
ODD-EVEN NUCLEI
ORGANS
RADIOISOTOPES
RADIONUCLIDE KINETICS
SECONDS LIVING RADIOISOTOPES
THALLIUM 201
THALLIUM ISOTOPES
UPTAKE
VERTEBRATES