Synthesis of protein in host-free reticulate bodies of Chlamydia psittaci and Chlamydia trachomatis

Journal Article · Sat Jun 01 00:00:00 EDT 1985 · J. Bacteriol.; (United States)

OSTI ID:6241210

Hatch, T P; Miceli, M; Silverman, J A

Synthesis of protein by the obligate intracellular parasitic bacteria Chlamydia psittaci (6BC) and Chlamydia trachomatis (serovar L2) isolated from host cells (host-free chlamydiae) was demonstrated for the first time. Incorporation of (/sup 35/S)methionine and (/sup 35/S)cysteine into trichloroacetic acid-precipitable material by reticulate bodies of chlamydiae persisted for 2 h and was dependent upon a exogenous source of ATP, an ATP-regenerating system, and potassium or sodium ions. Magnesium ions and amino acids stimulated synthesis; chloramphenicol, rifampin, oligomycin, and carbonyl cyanide p-trifluoromethoxyphenylhydrazone (a proton ionophore) inhibited incorporation. Ribonucleoside triphosphates (other than ATP) had little stimulatory effect. The optimum pH for host-free synthesis was between 7.0 and 7.5. The molecular weights of proteins synthesized by host-free reticulate bodies closely resembled the molecular weights of proteins synthesized by reticulate bodies in an intracellular environment, and included outer membrane proteins. Elementary bodies of chlamydiae were unable to synthesize protein even when incubated in the presence of 10 mM dithiothreitol, a reducing agent which converted the highly disulfide bond cross-linked major outer membrane protein to monomeric form.

• OSTI ID: 6241210
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 3; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

550701* -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
ALKALINE EARTH METAL COMPOUNDS
AMINO ACIDS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ATP
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
CARBOXYLIC ACIDS
CATIONS
CELL CONSTITUENTS
CELL MEMBRANES
CHARGED PARTICLES
CYCLOHEXIMIDE
CYSTEINE
DAYS LIVING RADIOISOTOPES
DRUGS
EVEN-ODD NUCLEI
FUNGICIDES
IONS
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAGNESIUM COMPOUNDS
MEMBRANES
METHIONINE
MICROORGANISMS
MOLECULAR WEIGHT
NUCLEI
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PESTICIDES
PH VALUE
POTASSIUM COMPOUNDS
PROTEINS
RADIOISOTOPES
SODIUM COMPOUNDS
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
THIOLS
TRACER TECHNIQUES