Extraskeletal and intraskeletal new bone formation induced by demineralized bone matrix combined with bone marrow cells
Dilutions of fresh autogenous bone marrow cells in combination with allogeneic demineralized cortical bone matrix were tested extraskeletally in rats using roentgenographic, histologic, and /sup 45/Ca techniques. Suspensions of bone marrow cells (especially diluted 1:2 with culture media) combined with demineralized cortical bone seemed to induce significantly more new bone than did demineralized bone, bone marrow, or composite grafts with whole bone marrow, respectively. In a short-term spinal fusion experiment, demineralized cortical bone combined with fresh bone marrow produced new bone and bridged the interspace between the spinous processes faster than other transplantation procedures. The induction of undifferentiated host cells by demineralized bone matrix is further complemented by addition of autogenous, especially slightly diluted, bone marrow cells.
- Research Organization:
- Orthopaedic Hospital of Invalid Foundation, Helsinki, Finland
- OSTI ID:
- 6240242
- Journal Information:
- Clin. Orthop.; (United States), Vol. 171
- Country of Publication:
- United States
- Language:
- English
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BONE TISSUES
BIOLOGICAL REGENERATION
CALCIUM 45
ISOTOPE APPLICATIONS
BIOCHEMICAL REACTION KINETICS
BONE MARROW CELLS
COMPARATIVE EVALUATIONS
GRAFTS
METABOLISM
RATS
ALKALINE EARTH ISOTOPES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL RECOVERY
BODY
CALCIUM ISOTOPES
CONNECTIVE TISSUE
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
INTERMEDIATE MASS NUCLEI
ISOTOPES
KINETICS
MAMMALS
NUCLEI
RADIOISOTOPES
REACTION KINETICS
RECOVERY
RODENTS
SOMATIC CELLS
TISSUES
TRANSPLANTS
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics