Stimulation of poly(ADP-ribose) synthetase activity in the lungs of mice exposed to a low level of ozone

Journal Article · Sun Sep 01 04:00:00 EDT 1985 · Arch. Biochem. Biophys.; (United States)

DOI:https://doi.org/10.1016/0003-9861(85)90573-9· OSTI ID:6240070

Hussain, M Z; Mustafa, M G; Ghani, Q P; Bhatnagar, R S

Toxic effects of O/sub 3/ are mediated through the formation of free radicals, which can cause DNA strand breaks. Cellular DNA repair is dependent upon the formation of poly(ADP-ribose) (polyADPR) catalyzed by polyADPR synthetase. In order to evaluate whether O/sub 3/ exposure inflicted DNA damage in lung tissue, the authors measured the activity of polyADPR synthetase (known to be activated in response to DNA damage) in mouse lungs after exposure to 0.45 ppm (882 micrograms/m3) O/sub 3/ for up to 7 days. The enzyme activity was stimulated with O/sub 3/ exposure relative to unexposed controls, showing a 20% (P less than 0.05) increase at Day 5 and 42% (P less than 0.001) at Day 7 of O/sub 3/ exposure. In addition, the activity of superoxide dismutase (SOD), known to be stimulated in response to production of superoxide anion (.O2-), was measured as an indicator of free radical involvement. Relative to unexposed controls, the SOD activity in exposed animal lungs increased to the peak level at Day 5 (48%, P less than 0.001) and then declined at Day 7 of O/sub 3/ exposure but was still higher than controls (17%, P less than 0.05). When animals, after 5 days of O/sub 3/ exposure, were allowed to recover in filtered room air, the activities of both enzymes declined to their respective control values in 6 days. These results suggest a possible temporal relationship between O/sub 3/ injury and the activities of polyADPR synthetase and a free radical scavenging enzyme, SOD. The stimulation of polyADPR synthetase activity with O/sub 3/ exposure, reflecting a response to lung cellular DNA repair, may be a sensitive indicator for assessing DNA damage in oxidant injury.

• Research Organization: Univ. of California, San Francisco
• OSTI ID: 6240070
• Journal Information: Arch. Biochem. Biophys.; (United States), Journal Name: Arch. Biochem. Biophys.; (United States) Vol. 2; ISSN ABBIA
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADP
AIR POLLUTION
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL INDICATORS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
COENZYMES
DNA REPAIR
ENZYME ACTIVITY
ENZYMES
GENETIC EFFECTS
LIGASES
LUNGS
MAMMALS
MICE
NAD
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
OZONE
POLLUTION
RECOVERY
REPAIR
RESPIRATORY SYSTEM
RIBOSIDES
RODENTS
STRAND BREAKS
SUPEROXIDE DISMUTASE
TOXICITY
VERTEBRATES