Relationship between the metabolism of haloacetonitriles and chloroform and their carcinogenic activity

Technical Report · Sat Sep 01 04:00:00 EDT 1984

OSTI ID:6239275

Pereira, M A; Daniel, F B; Lin, E L.C.

Halogenated acetonitriles, found in chlorinated drinking water, are formed by the reaction of chlorine with algae, fulvic acid, and proteinaceous material in the source water. The metabolism of halogenated acetonitriles was investigated in relation to their possible carcinogenic activity. Chloroacetonitrile (CAN), dibromoacetonitrile (DBAN), dichloroacetonitrile (DCAN), bromochloroacetonitrile (BCAN), and trichloroacetonitrile (TCAN) were shown to bind the nucleophilic trapping agent, p-nitrobenzylpyridine, without metabolic activation and to produce strand breaks in the DNA of cultured human CCRF-CEM cells. In addition, DCAN bound DNA in vitro without metabolic activation producing an adduct to adenine, and DBAN bound rat-liver DNA following oral administration.

• Research Organization: Environmental Protection Agency, Cincinnati, OH (USA). Health Effects Research Lab.
• OSTI ID: 6239275
• Report Number(s): PB-84-246230; EPA/600-D-84/203
• Country of Publication: United States
• Language: English

Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETONITRILE
ANIMAL CELLS
ANIMALS
CARCINOGEN SCREENING
CARCINOGENS
CELL CULTURES
CHEMICAL REACTIONS
CHLORINATED ALIPHATIC HYDROCARBONS
CHLORINATION
CHLOROFORM
HALOGENATED ALIPHATIC HYDROCARBONS
HALOGENATION
LABORATORY ANIMALS
MAMMALS
MAN
METABOLISM
NITRILES
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PRIMATES
SCREENING
STRAND BREAKS
VERTEBRATES