Formaldehyde metabolism by Escherichia coli. Carbon and solvent deuterium incorporation into glycerol, 1,2-propanediol, and 1,3-propanediol
Escherichia coli were grown on 14.3% uniformly TC-labeled glucose as the sole carbon source and challenged anaerobically with 90% TC-labeled formaldehyde. The major multiply labeled metabolites were identified by TC NMR spectroscopy to be glycerol and 1,2-propanediol, and a minor metabolite was shown to be 1,3-propanediol. In each case, formaldehyde is incorporated only into the C1 position. A novel form of TC NMR isotope dilution analysis of the major products reveals that all the 1,2-diol C1 is formaldehyde derived but that about 40% of the glycerol C1 is derived from bacterial sources. Glycerokinase converted the metabolite (1- TC)glycerol to equal amounts of (3- TC)glycerol 3-phosphate and (1- TC)glycerol 3-phosphate, demonstrating that the metabolite is racemic. When ( TC)formaldehyde incubation was carried out in H2O/D2O mixtures, deuterium incorporation was detected by beta- and gamma-isotope shifts. The 1,3-diol is deuterium labeled only at C2 and only once, while the 1,2-diol and glycerol are each labeled independently at both C2 and C3; C3 is multiply labeled. Deuterium incorporation levels are different for each metabolite, indicating that the biosynthetic pathways probably diverge early.
- Research Organization:
- Queen's Univ., Kingston, Ontario
- OSTI ID:
- 6234291
- Journal Information:
- Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 15; ISSN BICHA
- Country of Publication:
- United States
- Language:
- English
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62 RADIOLOGY AND NUCLEAR MEDICINE
ALCOHOLS
ALDEHYDES
BACTERIA
CARBOHYDRATES
CARBON 13
CARBON ISOTOPES
ESCHERICHIA COLI
EVEN-ODD NUCLEI
FORMALDEHYDE
GLUCOSE
GLYCOLS
HEAVY WATER
HEXOSES
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
METABOLISM
METABOLITES
MICROORGANISMS
MONOSACCHARIDES
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
RESONANCE
SACCHARIDES
SOLVENTS
STABLE ISOTOPES
TRACER TECHNIQUES
WATER