Microbial conversion of glycerol to 1,3-propanediol and related compounds
- Univ. of Wisconsin, Madison, WI (United States); and others
Glycerol is a co-product of the conversion of vegetable oils and animal fats to soap, fatty acids, fatty acid esters and biodiesel fuel. We have investigated the use of both native and recombinant microorganisms for the conversion of glycerol to more valuable products. Fed-batch fermentations of glycerol by Klebsiella pneumoniae gave over 50 g/L of 1,3-propanediol (1,3-PD, trimethylene glycol) with high volumetric productivity. Escherichia coli containing genes for glycerol dehydratase and 1,3-PD oxidoreductase from K. pneumoniae also gave 1,3-PD from glycerol, but at a lower level than the native organism. Experiments with the recombinant E. coli have provided insights into ways to improve 1,3-PD production in both the recombinant and native organism and to modify the pathway for the production of compounds such as 3-hydroxypropionaldehyde and dihydroxyacetone.
- OSTI ID:
- 127032
- Report Number(s):
- CONF-950402--
- Country of Publication:
- United States
- Language:
- English
Similar Records
Microbial conversion of glycerol to 1,3-propanediol
1,3-Propanediol production by Escherichia coli expressing genes from the Klebsiella pneumoniae dha regulon