Neutrophil kinetics of recombinant human granulocyte colony-stimulating factor-induced neutropenia in rats

Journal Article · Sun Dec 31 23:00:00 EST 1989 · Life Sciences; (USA)

DOI:https://doi.org/10.1016/0024-3205(90)90206-7· OSTI ID:6230393

Okada, Yuji; Kawagishi, Mayumi; Kusaka, Masaru ^[1]

---

1. Kirin Brewery Co., Ltd., Gunma (Japan)

Single injection of recombinant human granulocyte colony-stimulating factor (rhG-CSF) immediately induced a decrease in the number of circulating neutrophils in rats. This neutropenia occurred 10 minutes after the injection but disappeared 40 minutes after injection. This transient neutropenia was dose-dependently induced by rhG-CSF and also induced by repeated injections. We studied the kinetics of circulating neutrophils in transient neutropenia. rhG-CSF markedly decreased the number of {sup 3}H-diisopropylfluorophosphate ({sup 3}H-DFP) labeled neutrophils in the circulation 10 minutes after injection but the labeled neutrophils recovered to near the control level 40 minutes after the injection. These results indicate that the neutrophil margination accounts for the neutrophenia and the marginated neutrophils return to the circulation.

• OSTI ID: 6230393
• Journal Information: Life Sciences; (USA), Journal Name: Life Sciences; (USA) Vol. 47:15; ISSN 0024-3205; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOCHEMICAL REACTION KINETICS
DOSE-RESPONSE RELATIONSHIPS
GROWTH FACTORS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
MAMMALS
MAN
MITOGENS
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
RATS
REACTION KINETICS
RODENTS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES