Evaluation of a monoclonal antibody-based immunoradiometric assay for prostatic acid phosphatase

Journal Article · Sat Jan 01 04:00:00 EST 1983 · Am. J. Clin. Pathol.; (United States)

DOI:https://doi.org/10.1093/ajcp/79.1.114· OSTI ID:6224272

Davies, S N; Gochman, N

This report evaluates a new immunoradiometric assay for prostatic acid phosphatase in serum, based on a dual monoclonal antibody reaction system (Hybritech-TANDEM). A solidphase antibody binds the acid phosphatase molecule and a second monoclonal antibody to a different antigenic site serves as the /sup 125/I-radiolabel. The method was tested on 67 patients with various stages of prostatic carcinoma and 134 patients without the disease. It also was compared with a conventional polyclonal radioimmunoassay (NEN) and an enzymatic activity method (duPont aca). The upper limit for the TANDEM assay on nondiseased male patients was found to be 2.0 microgram/L. Based on this upper limit of normal, the diagnostic sensitivity of the method for all cases of prostatic carcinoma was 60%. Researchs could not distinguish the enzyme released in abnormal amounts due to benign prostatic hypertrophy and certain nonprostatic malignant diseases from that of prostatic carcinoma. The diagnostic specificity was calculated at 95%. For the clinically undetectable Stage 1 disease, sensitivity was 44% (four abnormal values out of nine cases). The TANDEM procedure is simple to use and reproducible.

• Research Organization: Laboratory Service, Veterans Administration Medical Center, San Diego, California
• OSTI ID: 6224272
• Journal Information: Am. J. Clin. Pathol.; (United States), Journal Name: Am. J. Clin. Pathol.; (United States) Vol. 79:1; ISSN AJCPA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ACID PHOSPHATASE
ANTIBODIES
BETA DECAY RADIOISOTOPES
BODY
CALCULATION METHODS
COMPARATIVE EVALUATIONS
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC USES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ENZYME ACTIVITY
ENZYMES
ESTERASES
GLANDS
HYDROLASES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MALE GENITALS
MONOCLONAL ANTIBODIES
NEOPLASMS
NORTH AMERICA
NUCLEI
ODD-EVEN NUCLEI
ORGANS
PATIENTS
PHOSPHATASES
PROSTATE
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
TRACER TECHNIQUES
USA
USES