New monoclonal-antibody two-site solid-phase immunoradiometric assay for human thyrotropin evaluated

Journal Article · Sun Jul 01 00:00:00 EDT 1984 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:6223025

Pekary, A E; Hershman, J M

The authors compared results with a commercial solid-phase two-site immunoradiometric assay kit for human thyrotropin in which monoclonal antibodies are used with those by our radioimmunoassay, which is optimized for measurement of low concentrations of thyrotropin. In the immunoradiometric assay a specific antibody to the beta subunit of human thyrotropin is immobilized on a polystyrene bead, and a radiolabeled monoclonal antibody directed against the alpha subunit provides a measure of bead-immobilized hormone. The mean thyrotropin concentrations in 70 euthyroid serum samples were similar in the two assays. Values for hypothyroid patients were clearly higher in both assays than values for euthyroid individuals. In commercial assays the major source of error in measurement of thyrotropin response to thyroliberin in terms of the increment over the basal concentration of thyrotropin has been systematic errors in the measurement of those basal concentrations. With the present assay, however, basal values are obtained with good precision and accuracy.

• Research Organization: Wadsworth Medical Center, Los Angeles, CA
• OSTI ID: 6223025
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Journal Name: Clin. Chem. (Winston-Salem, N.C.); (United States) Vol. 30:7; ISSN CLCHA
• Country of Publication: United States
• Language: English

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