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gamma. irradiation of aqueous solutions of human hemoglobin in atmospheres of air and argon

Journal Article · · Radiat. Res.; (United States)
DOI:https://doi.org/10.2307/3574966· OSTI ID:6221802

In this study, the degrees of destruction of hemoglobin irradiated in atmospheres of air and argon were compared. Hemoglobin preparations were irradiated in the forms: oxyhemoglobin (HbO/sub 2/) deoxyhemoglobin (Hb/sup 2 +/) and methemoglobin (MetHb) applying doses of 0.5 to 5 Mrad. The degree of hemoglobin destruction was estimate on the basis of changes in the values of the absorption coefficient at the Soret band, the absorption ratio A/sub 505//A/sub 563/ determined after conversion of irradiated preparations into MetHb, absorption coefficinets for pyridine hemochromogen obtained from irradiated preparations, and changes in parameters characterizing the hemoglobin oxygenation reaction (log p/sub 1/2/O/sub 2/ and the Hill n coefficient). The calculated oxygen enhancement ratios S were generally higher than 1 for the parameters estimated. This indicates that the presence of oxygen during irradiation enhances hemoglobin destruction.

Research Organization:
Univ. of Lodz, Poland
OSTI ID:
6221802
Journal Information:
Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 78:3; ISSN RAREA
Country of Publication:
United States
Language:
English

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