Effects of gamma irradiation on the structure and function of human hemoglobin
In this paper physicochemical and functional properties of irradiated aqueous hemoglobin solutions are presented. HbO$sub 2$ solutions (5 percent) were irradiated in air with doses ranging from 0.5 to 5 Mrad at the efficiency of 1 Mrad/hr. At doses exceeding 1 Mrad, coagulation of small amounts of protein was observed. Hemoglobin which remained in the solution consisted of a mixture of HbO$sub 2$ and MetHb. At doses of 4 and 5 Mrad the presence of hemochromogen was established. The appropriate hydrodynamic parameters--molecular weight, sedimentation constant, and limiting viscosity value--do not differ from the control values up to a dose of 1 Mrad. At greater doses sedimentation constants and the limiting viscosity number increase. The decrease in the values of the second virial coefficient and the electrophoretic behavior in polyacrylamide gel suggest some changes in the electric charge of the molecules. Simultaneously, a considerable part of the molecules exhibits an increased affinity for oxygen and a decreased heme--heme interaction. (auth)
- Research Organization:
- Univ., Lodz, Poland
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-33-026135
- OSTI ID:
- 4023709
- Journal Information:
- Radiat. Res., v. 65, no. 1, pp. 50-59, Journal Name: Radiat. Res., v. 65, no. 1, pp. 50-59; ISSN RAREA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560111* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Biochemicals--In Vitro
AQUEOUS SOLUTIONS
DOSE- RESPONSE RELATIONSHIPS
GAMMA RADIATION
IN VITRO
IRRADIATION
MOLECULAR WEIGHT
N48110* --Life Sciences--Radiation Effects on Biochemicals-- In-Vitro
SEDIMENTATION
VISCOSITY