Proliferation of human melanoma cells after single and fractionated exposure to hyperthermia and irradiation
Journal Article
·
· NIDA Res. Monogr.; (United States)
OSTI ID:6220776
The growth, DNA synthesis, distribution of cells in the cell cycle, labeling index, and the formation of micronuclei were measured in a human melanoma cell line after single and fractionated treatment with hyperthermia (three times for 1 hr at 42 degrees C) and X-irradiation (4.0 gray three times) or a combination of both treatments. After fractionation with heat or irradiation alone, the cells showed a greater capacity for recovery than after fractionation with the combined modality. The DNA synthesis returned to the control values after each fraction at later periods. Concomitant with these observations was a decrease of labeled or unlabeled S-phase cells. The effect was more pronounced after the combined fractionation. Hypoploid cells as potentially dead cells were observed after each treatment, but hyperploid cells were observed only after the combined fractionation. According to the cell loss and occurrence of hypoploid cells, micronuclei increased except after heat alone.
- Research Organization:
- Institut fuer Medizinische Strahlenphysik und Strahlenbiologie, Universitaetsklinikum Essen, Federal Republic of Germany
- OSTI ID:
- 6220776
- Journal Information:
- NIDA Res. Monogr.; (United States), Journal Name: NIDA Res. Monogr.; (United States) Vol. 61; ISSN MIDAD
- Country of Publication:
- United States
- Language:
- English
Similar Records
Determination of potential doubling times in human melanoma cell cultures subjected to irradiation and/or hyperthermia by flow cytometry
Cell cycle-dependent expression of Ki-67 antigen in human melanoma cells subjected to irradiation and/or hyperthermia
Combined effects of X rays, Ro 03-8799, and hyperthermia on growth, necrosis, and cell proliferation in a mouse tumor
Journal Article
·
Wed Jun 01 00:00:00 EDT 1994
· Radiation Research
·
OSTI ID:79324
Cell cycle-dependent expression of Ki-67 antigen in human melanoma cells subjected to irradiation and/or hyperthermia
Journal Article
·
Sat Jul 01 00:00:00 EDT 1995
· Radiation Research
·
OSTI ID:121710
Combined effects of X rays, Ro 03-8799, and hyperthermia on growth, necrosis, and cell proliferation in a mouse tumor
Journal Article
·
Fri Mar 31 23:00:00 EST 1989
· Int. J. Radiat. Oncol., Biol. Phys.; (United States)
·
OSTI ID:6254506
Related Subjects
550300 -- Cytology
550400 -- Genetics
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY TEMPERATURE
CELL CONSTITUENTS
CELL CULTURES
CELL CYCLE
CELL DIVISION
CELL NUCLEI
COMPARATIVE EVALUATIONS
DISEASES
DNA
ELECTROMAGNETIC RADIATION
FRACTIONATED IRRADIATION
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
HYPERTHERMIA
IONIZING RADIATIONS
IRRADIATION
MELANOMAS
MITOSIS
NEOPLASMS
NORTH AMERICA
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY EFFECTS
RESPONSE MODIFYING FACTORS
SYNERGISM
TUMOR CELLS
USA
X RADIATION
550400 -- Genetics
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY TEMPERATURE
CELL CONSTITUENTS
CELL CULTURES
CELL CYCLE
CELL DIVISION
CELL NUCLEI
COMPARATIVE EVALUATIONS
DISEASES
DNA
ELECTROMAGNETIC RADIATION
FRACTIONATED IRRADIATION
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
HYPERTHERMIA
IONIZING RADIATIONS
IRRADIATION
MELANOMAS
MITOSIS
NEOPLASMS
NORTH AMERICA
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY EFFECTS
RESPONSE MODIFYING FACTORS
SYNERGISM
TUMOR CELLS
USA
X RADIATION