Cell cycle-dependent expression of Ki-67 antigen in human melanoma cells subjected to irradiation and/or hyperthermia
- Universitaetsklinikum Essen (Germany)
The proliferation of human melanoma cells in vitro during the first 3 days after irradiation and/or hyperthermia was followed by two-parameter flow cytometry combining cell cycle analysis on the basis of DNA content with Ki-67 antibody labeling. It was found that cells arrested or delayed in the S and G{sub 2} phases of the cell cycle were Ki-67-positive in spite of the antigen`s very short half-life. Thus Ki-67 staining failed to reflect those changes in cell proliferation which typically occur in the course of a fractionated radiotherapy as well as those expected in the case of hyperthermia or a combined treatment. 24 refs., 3 figs., 1 tab.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 121710
- Journal Information:
- Radiation Research, Journal Name: Radiation Research Journal Issue: 1 Vol. 143; ISSN 0033-7587; ISSN RAREAE
- Country of Publication:
- United States
- Language:
- English
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