In vivo determination of the pyridine nucleotide reduction charge by carbon-13 nuclear magnetic resonance spectroscopy

Journal Article · Fri Oct 07 00:00:00 EDT 1983 · Science (Washington, D.C.); (United States)

DOI:https://doi.org/10.1126/science.6353573· OSTI ID:6220339

Unkefer, C J; Blazer, R M; London, R E

An intracellular coenzyme has been observed by carbon-13 nuclear magnetic resonance spectroscopy. The pyridine nucleotides in Escherichia coli were specifically labeled with carbon-13 from the biosynthetic precursor, nicotinic acid. The intracellular redox status and metabolic transformations of the pyridine nucleotides were examined under a variety of conditions. A highly reduced nicotinamide adenine dinucleotide pool was observed under anaerobic conditions only in cells that were cultured aerobically on glycerol.

• Research Organization: Los Alamos National Lab., NM
• OSTI ID: 6220339
• Journal Information: Science (Washington, D.C.); (United States), Vol. 222
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CARBON 13
NUCLEAR MAGNETIC RESONANCE
ESCHERICHIA COLI
METABOLISM
BIOCHEMICAL REACTION KINETICS
ENZYME ACTIVITY
NICOTINIC ACID
LABELLED COMPOUNDS
NUCLEOTIDES
LABELLING
PYRIDINE
REDOX REACTIONS
IN VIVO
AZINES
BACTERIA
CARBON ISOTOPES
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
EVEN-ODD NUCLEI
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
ISOTOPES
KINETICS
LIGHT NUCLEI
MAGNETIC RESONANCE
MICROORGANISMS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIDINES
REACTION KINETICS
RESONANCE
STABLE ISOTOPES
VITAMIN B GROUP
VITAMINS
560302* - Chemicals Metabolism & Toxicology- Microorganisms- (-1987)