In vivo determination of the pyridine nucleotide reduction charge by carbon-13 nuclear magnetic resonance spectroscopy

Journal Article · Fri Oct 07 04:00:00 EDT 1983 · Science (Washington, D.C.); (United States)

DOI:https://doi.org/10.1126/science.6353573· OSTI ID:6220339

Unkefer, C J; Blazer, R M; London, R E

An intracellular coenzyme has been observed by carbon-13 nuclear magnetic resonance spectroscopy. The pyridine nucleotides in Escherichia coli were specifically labeled with carbon-13 from the biosynthetic precursor, nicotinic acid. The intracellular redox status and metabolic transformations of the pyridine nucleotides were examined under a variety of conditions. A highly reduced nicotinamide adenine dinucleotide pool was observed under anaerobic conditions only in cells that were cultured aerobically on glycerol.

• Research Organization: Los Alamos National Lab., NM
• OSTI ID: 6220339
• Journal Information: Science (Washington, D.C.); (United States), Journal Name: Science (Washington, D.C.); (United States) Vol. 222; ISSN SCIEA
• Country of Publication: United States
• Language: English

Related Subjects

560302* -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AZINES
BACTERIA
BIOCHEMICAL REACTION KINETICS
CARBON 13
CARBON ISOTOPES
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
ENZYME ACTIVITY
ESCHERICHIA COLI
EVEN-ODD NUCLEI
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
IN VIVO
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LABELLING
LIGHT NUCLEI
MAGNETIC RESONANCE
METABOLISM
MICROORGANISMS
NICOTINIC ACID
NUCLEAR MAGNETIC RESONANCE
NUCLEI
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIDINE
PYRIDINES
REACTION KINETICS
REDOX REACTIONS
RESONANCE
STABLE ISOTOPES
VITAMIN B GROUP
VITAMINS