In vivo determination of the pyridine nucleotide reduction charge by carbon-13 nuclear magnetic resonance spectroscopy

Journal Article · Fri Oct 07 00:00:00 EDT 1983 · Science (Washington, D.C.); (United States)

DOI:https://doi.org/10.1126/science.6353573· OSTI ID:5663280

Unkefer, C J; Blazer, R M; London, R E

An intracellular coenzyme has been observed by carbon-13 nuclear magnetic resonance spectroscopy. The pyridine nucleotides in Escherichia coli were specifically labeled with carbon-13 from the biosynthetic precursor, nicotinic acid. The intracellular redox status and metabolic transformations of the pyridine nucleotides were examined under a variety of conditions. A highly reduced nicotinamide adenine dinucleotide pool was observed under anaerobic conditions only in cells that were cultured aerobically on glycerol.

• Research Organization: Los Alamos National Lab., NM
• OSTI ID: 5663280
• Journal Information: Science (Washington, D.C.); (United States), Vol. 222
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CARBON 13
NUCLEAR MAGNETIC RESONANCE
NAD
LABELLED COMPOUNDS
LABELLED POOL TECHNIQUES
METABOLISM
REDUCTION
NADP
ESCHERICHIA COLI
IN VIVO
REDOX REACTIONS
BACTERIA
CARBON ISOTOPES
CHEMICAL REACTIONS
COENZYMES
EVEN-ODD NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MICROORGANISMS
NUCLEI
NUCLEOTIDES
ORGANIC COMPOUNDS
RESONANCE
STABLE ISOTOPES
TRACER TECHNIQUES
550200* - Biochemistry