In vivo determination of the pyridine nucleotide reduction charge by carbon-13 nuclear magnetic resonance spectroscopy

Journal Article · Fri Oct 07 04:00:00 EDT 1983 · Science (Washington, D.C.); (United States)

DOI:https://doi.org/10.1126/science.6353573· OSTI ID:5663280

Unkefer, C J; Blazer, R M; London, R E

An intracellular coenzyme has been observed by carbon-13 nuclear magnetic resonance spectroscopy. The pyridine nucleotides in Escherichia coli were specifically labeled with carbon-13 from the biosynthetic precursor, nicotinic acid. The intracellular redox status and metabolic transformations of the pyridine nucleotides were examined under a variety of conditions. A highly reduced nicotinamide adenine dinucleotide pool was observed under anaerobic conditions only in cells that were cultured aerobically on glycerol.

• Research Organization: Los Alamos National Lab., NM
• OSTI ID: 5663280
• Journal Information: Science (Washington, D.C.); (United States), Journal Name: Science (Washington, D.C.); (United States) Vol. 222; ISSN SCIEA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
CARBON 13
CARBON ISOTOPES
CHEMICAL REACTIONS
COENZYMES
ESCHERICHIA COLI
EVEN-ODD NUCLEI
IN VIVO
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LABELLED POOL TECHNIQUES
LIGHT NUCLEI
MAGNETIC RESONANCE
METABOLISM
MICROORGANISMS
NAD
NADP
NUCLEAR MAGNETIC RESONANCE
NUCLEI
NUCLEOTIDES
ORGANIC COMPOUNDS
REDOX REACTIONS
REDUCTION
RESONANCE
STABLE ISOTOPES
TRACER TECHNIQUES