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Gene for the human immune interferon receptor is located on chromosome 6

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
; ; ; ; ; ; ; ;
When /sup 32/P-labeled human recombinant immune interferon ..gamma.. (Hu-(/sup 32/P)IFN-..gamma..) is crosslinked to human cells with disuccinimidyl suberate, a complex with a molecular size of approx.117,000 Da was identified by sodium dodecyl sulfate/polyacrylamide gel electrophoresis. The formation of this complex is inhibited when the binding is performed in the presence of excess unlabeled Hu-IFN-..gamma... The specific formation of the 117,000-Da complex is not observed in mouse L cells or Chinese hamster ovary cells. This complex shows all of the criteria that identify it as the Hu-IFN-..gamma.. receptor or its binding subunit. The same complex can be formed following binding and covalent crosslinking of Hu-(/sup 32/P)IFN-..gamma.. to some hamster-human or mouse-human somatic cell hybrids. The presence of human chromosome 6 in the hybrids is necessary and sufficient for the formation of this complex. More specifically, the long arm of chromosome 6 seems sufficient. Therefore, the authors have localized the gene for the Hu-IFN-..gamma.. receptor (or its binding subunit) to the long arm of human chromosome 6. The presence of this chromosome in the somatic cell hybrids is not adequate, however, to confer antiviral resistance to the hybrids in the presence of Hu-IFN-..gamma...
Research Organization:
Roche Institute of Molecular Biology, Nutley, NJ
OSTI ID:
6220238
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 83:2; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ATP
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHEMICAL REACTIONS
CHO CELLS
CHROMOSOMES
CONNECTIVE TISSUE CELLS
CROSS-LINKING
GENES
GENETIC MAPPING
GROWTH FACTORS
INTERFERON
LABELLED COMPOUNDS
LEUKOCYTES
LYMPHOCYTES
LYMPHOKINES
MAMMALS
MAN
MAPPING
MATERIALS
MEMBRANE PROTEINS
MICE
MITOGENS
NUCLEOTIDES
ORGANIC COMPOUNDS
POLYMERIZATION
PRIMATES
PROTEINS
RECEPTORS
RODENTS
SOMATIC CELLS
VERTEBRATES