Genetic relatedness of Legionella longbeachae isolates from human and environmental sources in Australia

Journal Article · Sat Sep 01 00:00:00 EDT 1990 · Applied and Environmental Microbiology; (USA)

OSTI ID:6217476

Lanser, J A; Doyle, R; Sangster, N; Steele, T W ^[1]; Adams, M ^[2]

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1. Institute of Medical and Veterinary Science, From Road, Adelaide (Australia)
2. South Australian Museum, Adelaide (Australia)

The genetic relatedness of Legionella longbeachae isolated in Australia since 1987 was investigated by restriction fragment length polymorphism (RFLP) analysis and allozyme electrophoresis. Three radiolabeled probes were used in Southern hybridizations for the RFLP studies. They were Escherichia coli 16S and 23S rRNA and cloned fragments of L. longbeachae selected empirically from genomal banks in lambda and a cosmid. The legionellae included in the study comprised 11 Legionella longbeachae serogroup 1 organisms isolated form humans, 28 L. longbeachae serogroup 1 isolates from environmental sources, 3 L. longbeachae serogroup 2 environmental isolates. These were compared with the American Type Culture Collection reference strains of both serogroups and some other related Legionella species. Results of allozyme and RFLP analysis showed that all the isolates from humans and all but three of the environmental L. longbeachae serogroup 1 isolates were closely related. They were also closely related to L. longbeachae serogroup 1 ATCC 33462. There was wider variation among the three L. longbeachae serogroup 2 environmental isolates. One of these was closely related to L. longbeachae serogroup 2 ATCC 33484. RFLP studies with the rRNA probe provided the most discrimination among isolates but did not distinguish between the two serogroups.

• OSTI ID: 6217476
• Journal Information: Applied and Environmental Microbiology; (USA), Vol. 56:9; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BACTERIA
GENETIC VARIABILITY
AUSTRALIA
COMPARATIVE EVALUATIONS
DNA HYBRIDIZATION
ELECTROPHORESIS
ENVIRONMENT
ESCHERICHIA COLI
MAN
RFLPS
RNA
TRACER TECHNIQUES
ANIMALS
AUSTRALASIA
BIOLOGICAL VARIABILITY
HYBRIDIZATION
ISOTOPE APPLICATIONS
MAMMALS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PRIMATES
VERTEBRATES
550401* - Genetics- Tracer Techniques