Legionella anisa: a new species of Legionella isolated from potable waters and a cooling tower
Between March 1980 and June 1981, five strains of Legionella-like organisms were isolated from water. Four were recovered from potable water collected from hospitals in Chicago, IL, and Los Angeles, CA, during outbreaks of nosocomial legionellosis. The fifth strain was isolated from water collected from an industrial cooling tower in Jamestown, NY. The strains exhibited biochemical reactions typical of Legionella species and were gram-negative motile rods which grew on buffered charcoal-yeast extract agar but not on blood agar, required cysteine, and were catalase positive, urease negative, nitrate negative, hippurate negative, and nonfermentative. All strains were positive for oxidase and beta-lactamase and produced a brown, diffusible pigment. The fatty-acid composition and ubiquinone content of these strains were consistent with those of other Legionella species. Direct fluorescent-antibody examination of the five strains with conjugates to previously described Legionella species demonstrated no cross-reactions except with the conjugates to L. longbeachae serogroup 2 and L. bozemannii serogroup 2. Four strains gave a 4+ reaction to the L. longbeachae serogroup 2 conjugate and the fifth strain gave a 1+ reaction. Each of the five strains gave a 4+ reaction with the conjugate to L. bozemanii serogroup 2. DNAs from the five strains were highly related (84 to 99%) and showed 5 to 57% relatedness to other Legionella species. These strains constitute a new species in the genus Legionella, and the name Legionella anisa sp. nov. is proposed.
- Research Organization:
- Centers for Disease Control, Atlanta, GA
- OSTI ID:
- 5777541
- Journal Information:
- Appl. Environ. Microbiol.; (United States), Vol. 49:2
- Country of Publication:
- United States
- Language:
- English
Similar Records
Legionella spp. in Puerto Rico cooling towers
Legionella in Puerto Rico cooling towers
Related Subjects
COOLING SYSTEMS
CONTAMINATION
DRINKING WATER
LEGIONELLA ANISA
CHEMICAL COMPOSITION
BACTERIAL DISEASES
BIOCHEMICAL REACTION KINETICS
CHICAGO
CLASSIFICATION
FLUORESCENCE
GROWTH
INFECTIOUS DISEASES
LOS ANGELES
NEW YORK
TAXONOMY
BACTERIA
BIOLOGY
CALIFORNIA
DISEASES
ENERGY SYSTEMS
FEDERAL REGION II
FEDERAL REGION IX
FEDERAL REGION V
HYDROGEN COMPOUNDS
ILLINOIS
KINETICS
LUMINESCENCE
MICROORGANISMS
NORTH AMERICA
OXYGEN COMPOUNDS
REACTION KINETICS
USA
WATER
550700* - Microbiology