Cerebellar degeneration-related antigen: A highly conserved neuroectodermal marker mapped to chromosomes X in human and mouse

Journal Article · Sun Apr 01 04:00:00 EDT 1990 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.87.8.3077· OSTI ID:6213689

Chen, Yao-Tseng; Rettig, W J; Yenamandra, A K; Chaganti, R S.K.; Posner, J B; Old, L J ^[1]; Kozak, C A ^[2]

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1. Memorial Sloan-Kettering Cancer Center, New York, NY (USA)
2. National Institutes of Health, Bethesda, MD (USA)

Cerebellar degeneration-related antigen (designated CDR34) was previously cloned by antibody screening of a cDNA library and was shown to be one of the target molecules recognized by autoantibodies in patients with paraneoplastic cerebellar degeneration. This molecule is distinctive in that it contains a tandem hexapeptide repetitive structure, presumable the basis for its high immunogenicity. In this study, the authors cloned the human CDR34 gene and proved that the entire repetitive sequence is encoded by a single exon without introns. They also showed that the nucleotide repeats are preserved only in the protein-coding sequences, suggesting evolutionary constraint in this region of the gene. Corresponding mouse cDNA clones were also isolated, which encoded a larger molecule with very similar hexapeptide repeating units. Comparison of the human and mouse repeats revealed a highly conserved Glu-Asp core in each unit, implicating the functional significance of this motif. Chromosomal mapping by somatic cell hybrid analysis mapped CDR34 to both human and mouse chromosomes X, and in situ hybridization further assigned CDR34 to human Xq24-q27.

• OSTI ID: 6213689
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 87:8; ISSN PNASA; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMALS
ANTIGENS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MARKERS
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
CEREBELLUM
CHROMOSOMES
DAYS LIVING RADIOISOTOPES
DISEASES
DNA
DNA HYBRIDIZATION
DNA SEQUENCING
GENETIC MAPPING
HEREDITARY DISEASES
HETEROCHROMOSOMES
HUMAN X CHROMOSOME
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
MAMMALS
MAPPING
METABOLIC DISEASES
MICE
MOLECULAR STRUCTURE
NERVOUS SYSTEM
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PATIENTS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
RADIOISOTOPES
RECOMBINANT DNA
RODENTS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
X CHROMOSOME