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Cloning of a brain protein identified by autoantibodies from a patient with paraneoplastic cerebellar degeneration

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
; ; ;
Autoantibodies directed against neuronal proteins have been identified in some patient with paraneoplastic cerebellar degeneration. To identify the molecular targets for these autoantibodies, the authors constructed a lambdagt11 cDNA expression library from human cerebellum and screened the library with IgG from a patient with paraneoplastic cerebellar degeneration. A single clone, pCDR2, produced a fusion protein that reacted strongly with the patient's IgG. The isolated pCDR2 clone was used to identify six overlapping cDNA clones. Sequencing of the pCDR clones revealed a distinctive pattern consisting of a unit of 18 nucleotides (6 amino acids) repeated in tandem along the entire cDNA sequence. This sequence is unlikely any previously described eukaryotic gene. Southern blot analysis was consistent with single-copy representation of the CDR (cerebellar degeneration-related) gene in the human and mouse genome. RNA blotting studies with normal tissues showed expression of the CDR gene to be largely restricted to brain. Expression of the CDR message was also noted in cell lines derived from cancers of neuroectodermal, kidney, and lung origin.
Research Organization:
Memorial Sloan-Kettering Cancer Center, New York, NY (USA)
OSTI ID:
5409685
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:13; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
550601 -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIBODIES
AUTORADIOGRAPHY
BIOLOGY
BODY
BRAIN
CARDIOVASCULAR SYSTEM
CENTRAL NERVOUS SYSTEM
CEREBELLUM
DIGESTIVE SYSTEM
DISEASES
DNA
DNA SEQUENCING
ELECTROPHORESIS
ETIOLOGY
GENETICS
GLANDS
GLOBULINS
HEART
IMMUNOGLOBULINS
KIDNEYS
LIVER
LUNGS
MAMMALS
MICE
NEOPLASMS
NERVOUS SYSTEM
NERVOUS SYSTEM DISEASES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PATIENTS
PROTEINS
RECOMBINANT DNA
RESPIRATORY SYSTEM
RODENTS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES