Human genes for the. alpha. and. beta. chains of complement C4b-binding protein are closely linked in a head-to-tail arrangement

Journal Article · Fri Jun 01 04:00:00 EDT 1990 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.87.12.4529· OSTI ID:6213675

Pardo-Manuel, F; Rey-Campos, J; De Cordoba, S R ^[1]; Hillarp, A; Dahlbaeck, B ^[2]

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1. Consejo Superior de Investigaciones Cientificas, Madrid (Spain)
2. Univ. of Lund, Malmo (Sweden)

C4b-binding protein (C4BP) is an important component in the regulation of the complement system and also binds the anticoagulant vitamin K-dependent protein S. These activities are performed by distinct, although structurally related, polypeptides of 70 kDa ({alpha} chain) and 45 kDa ({beta} chain), respectively. In this report the authors have investigated the genetic relationships between these polypeptides. Using pulsed field gel electrophoresis analysis they demonstrate that the genes coding for the {alpha} (C4BP{alpha}) and {beta} (C4BP{beta}) chains are closely linked within the regulator of complement activation gene cluster. In addition, they have determined that the 3{prime} end of the C4BP{beta} gene lies 3.5-5 kilobases from the 5{prime} end of the C4BP{alpha} gene. These findings support the concept that the C4BP{alpha} and C4BP{beta} genes are the result of a gene duplication event.

• OSTI ID: 6213675
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 87:12; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AUTORADIOGRAPHY
COMPLEMENT
DNA
ELECTROPHORESIS
GENE REGULATION
GENES
GENETIC MAPPING
MAPPING
MEMBRANE PROTEINS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PEPTIDES
POLYPEPTIDES
PROTEINS