Use of a simian virus 40-based shuttle vector to analyze enhanced mutagenesis in mitomycin C-treated monkey cells
Journal Article
·
· Mol. Cell. Biol.; (United States)
When monkey cells were treated with mitomycin C 24 h before transfection with UV-irradiated pZ189 (a simian virus 40-based shuttle vector), there was a twofold increase in the frequency of mutations in the supF gene of the vector. These results suggest the existence of an enhancible mutagenesis pathway in mammalian cells. However, DNA sequence analysis of the SupF- mutants suggested no dramatic changes in the mechanisms of mutagenesis due to mitomycin C treatment of the cells.
- Research Organization:
- National Institute of Child Health and Human Development, Bethesda, MD (USA)
- OSTI ID:
- 6212366
- Journal Information:
- Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 8:9; ISSN MCEBD
- Country of Publication:
- United States
- Language:
- English
Similar Records
Error-prone mutagenesis detected in mammalian cells by a shuttle vector containing the supF gene of Escherichia coli
Enhanced mutagenesis of UV-irradiated simian virus 40 occurs in mitomycin C-treated host cells only at a low multiplicity of infection
Sequence specificity of point mutations induced during passage of a UV-irradiated shuttle vector plasmid in monkey cells
Journal Article
·
Mon Oct 01 00:00:00 EDT 1984
· Mol. Cell. Biol.; (United States)
·
OSTI ID:5877926
Enhanced mutagenesis of UV-irradiated simian virus 40 occurs in mitomycin C-treated host cells only at a low multiplicity of infection
Journal Article
·
Mon Mar 31 23:00:00 EST 1986
· Mol. Cell. Biol.; (United States)
·
OSTI ID:6928028
Sequence specificity of point mutations induced during passage of a UV-irradiated shuttle vector plasmid in monkey cells
Journal Article
·
Tue Dec 31 23:00:00 EST 1985
· Mol. Cell. Biol.; (United States)
·
OSTI ID:6841257
Related Subjects
560130* -- Radiation Effects on Microorganisms
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
BIOLOGICAL EFFECTS
BIOLOGICAL PATHWAYS
DISEASE VECTORS
DNA SEQUENCING
DRUGS
ELECTROMAGNETIC RADIATION
GENES
MAMMALS
MICROORGANISMS
MITOMYCIN
MONKEYS
MUTAGENESIS
MUTATION FREQUENCY
PARASITES
PRIMATES
RADIATIONS
SIMIAN VIRUS
STRUCTURAL CHEMICAL ANALYSIS
ULTRAVIOLET RADIATION
VERTEBRATES
VIRUSES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
BIOLOGICAL EFFECTS
BIOLOGICAL PATHWAYS
DISEASE VECTORS
DNA SEQUENCING
DRUGS
ELECTROMAGNETIC RADIATION
GENES
MAMMALS
MICROORGANISMS
MITOMYCIN
MONKEYS
MUTAGENESIS
MUTATION FREQUENCY
PARASITES
PRIMATES
RADIATIONS
SIMIAN VIRUS
STRUCTURAL CHEMICAL ANALYSIS
ULTRAVIOLET RADIATION
VERTEBRATES
VIRUSES