Error-prone mutagenesis detected in mammalian cells by a shuttle vector containing the supF gene of Escherichia coli

Journal Article · Mon Oct 01 00:00:00 EDT 1984 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.4.10.2227· OSTI ID:5877926

Sarkar, S; Dasgupta, U B; Summers, W C

When a shuttle vector containing a tyrosine suppressor tRNA (supF) gene as a target for mutagenesis replicated in a monkey kidney cell line, the frequency of SupF/sup +/ mutations was 2.3 +- 0.5 x 10/sup -3/. When the host cells were treated with ethyl methanesulfonate 40 h before transfection, a 10-fold increase in SupF/sup +/ mutation frequency was observed. These results supported the hypothesis that a damage-inducible mutagenic pathway exists in mammalian cells and also demonstrated the utility of this shuttle vector for the study of mutagenesis in mammalian cells.

• Research Organization: Departments of Therapeutic Radiology, Molecular Biophysics and Biochemistry, and Human Genetics, Yale Univ. School of Medicine, New Haven, CT 06510
• OSTI ID: 5877926
• Journal Information: Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 4:10; ISSN MCEBD
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
BIOLOGICAL PATHWAYS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
CARBOXYLIC ACIDS
CELL CULTURES
DNA REPAIR
DNA REPLICATION
EMS
ENZYME INDUCTION
ERRORS
ESTERS
GENE REGULATION
GENES
HOST-CELL REACTIVATION
HYDROXY ACIDS
HYPOTHESIS
KIDNEYS
MAMMALS
MONKEYS
MUTAGENESIS
MUTAGENS
MUTATION FREQUENCY
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PRIMATES
RECOVERY
REPAIR
RNA
STRAND BREAKS
SULFONIC ACID ESTERS
TOXICITY
TRANSFER RNA
TYROSINE
VERTEBRATES