Study of the repair of potentially lethal and sublethal radiation damage in Chinese hamster cells exposed to extremely hypo- or hypertonic NaCl solutions. [X rays]
The effect of 0.05 and 1.5 M NaCl solutions on the repair of potentially lethal damage (PLD) and sublethal damage (SLD) in irradiated Chinese hamster ovary (CHO) cells has been examined. A 20-min exposure to the 0.05 or 1.5 M salt solutions which was terminated immediately before irradiation had no effect on the repair of PLD. However, salt treatment during or after irradiation caused an inhibition of repair of PLD. The possibility that 0.05 or 1.5 M treatment irreversibly affected repair enzymes could be ruled out. Exposure of cells to 0.05 or 1.5 M NaCl solutions before or during the first x-ray dose of a split-dose regimen decreased the ability of the cell to repair SLD. The recovery observed in the experiments was characterized by a change in D/sub 0/ of the survival curves and was related to the loss of interaction of radiation-induced lesions and salt effects as the incubation time between the second x-ray dose and salt treatment increased. However, a partial return of the shoulder of the survival curve when salt treatment preceded split-dose irradiation indicated that some repair of SLD does occur. In summary, salt treatment prior to irradiation does not inhibit repair of either SLD or PLD, whereas, salt treatment during or after irradiation inhibits repair of both SLD and PLD.
- Research Organization:
- Colorado State Univ., Fort Collins
- OSTI ID:
- 6210429
- Journal Information:
- Radiat. Res.; (United States), Vol. 77:2
- Country of Publication:
- United States
- Language:
- English
Similar Records
Are three forms of potentially lethal damage expressed after X irradiation by treatment with hypertonic solutions in Chinese hamster V79 cells?
Shape of dose-survival curves for mammalian cells and repair of potentially lethal damage analyzed by hypertonic treatment
Related Subjects
ANIMAL CELLS
SURVIVAL TIME
BIOLOGICAL REPAIR
INHIBITION
DNA
RADIATION INJURIES
RADIOSENSITIVITY EFFECTS
TIME DEPENDENCE
SODIUM CHLORIDES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
ACUTE IRRADIATION
CELL CULTURES
EXPERIMENTAL DATA
FRACTIONATED IRRADIATION
HAMSTERS
HYPERTONIC SOLUTIONS
ISOLATED VALUES
LETHAL IRRADIATION
SUBLETHAL IRRADIATION
SURVIVAL CURVES
X RADIATION
ACUTE EXPOSURE
ALKALI METAL COMPOUNDS
ANIMALS
BIOLOGICAL RECOVERY
CHLORIDES
CHLORINE COMPOUNDS
DATA
DATA FORMS
DISPERSIONS
ELECTROMAGNETIC RADIATION
HALIDES
HALOGEN COMPOUNDS
INFORMATION
INJURIES
IONIZING RADIATIONS
IRRADIATION
MAMMALS
MIXTURES
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
RODENTS
SODIUM COMPOUNDS
SOLUTIONS
VERTEBRATES
560121* - Radiation Effects on Cells- External Source- (-1987)