Detection and characterization of novel metal-binding proteins. First year report, 1984-1985
Technical Report
·
OSTI ID:6208213
We have synthesized several model peptides based on the metallothionein (MT) protein sequence, and have used these, in conjunction with several naturally occurring proteins, to evaluate current methodologies for isolating and characterizing metal-binding proteins. Our off-contract research has indicated that High Pressure Liquid chromatography (HPLC) offers several distinct advantages compared to other techniques in this area. In addition, we have developed improved on-line analysis for the detection of sulfhydryl-containing proteins that have been separated by HPLC. 12 figs., 2 tabs.
- Research Organization:
- General Electric Co., Schenectady, NY (USA). Corporate Research and Development Dept.
- DOE Contract Number:
- FG02-84ER13278
- OSTI ID:
- 6208213
- Report Number(s):
- DOE/ER/13278-1; ON: DE86003749
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CHEMICAL PREPARATION
CHROMATOGRAPHY
CYSTEINE
DATA
DAYS LIVING RADIOISOTOPES
ELECTROPHORESIS
EVEN-ODD NUCLEI
EXPERIMENTAL DATA
INFORMATION
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIQUID COLUMN CHROMATOGRAPHY
METALLOPROTEINS
METALLOTHIONEIN
MOLECULAR STRUCTURE
NUCLEI
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PEPTIDES
PROTEINS
RADIOISOTOPES
SEPARATION PROCESSES
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
THIOLS
TRACER TECHNIQUES
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CHEMICAL PREPARATION
CHROMATOGRAPHY
CYSTEINE
DATA
DAYS LIVING RADIOISOTOPES
ELECTROPHORESIS
EVEN-ODD NUCLEI
EXPERIMENTAL DATA
INFORMATION
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIQUID COLUMN CHROMATOGRAPHY
METALLOPROTEINS
METALLOTHIONEIN
MOLECULAR STRUCTURE
NUCLEI
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PEPTIDES
PROTEINS
RADIOISOTOPES
SEPARATION PROCESSES
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
THIOLS
TRACER TECHNIQUES