Copper-binding protein in Mimulus guttatus
Conference
·
OSTI ID:5520836
A Cu-binding protein has been purified from the roots of Mimulus guttatus using gel permeation chromatography on Sephadex G-75 and anion exchange chromatography on DEAE Biogel A. The protein has similar properties to putative metallothioneins (MTS) purified from other angiosperms. Putative MT was estimated by measuring the relative percentage incorporation of (/sup 35/S) into fractions containing the protein after HPLC on SW 3000-gel. In the roots of both Cu-tolerant and non tolerant plants synthesis of putative MT is induced by increased Cu concentration in the nutrient solution. The relative percentage incorporation of (/sup 35/S) into putative MT is significantly higher in extracts from the roots of Cu-tolerant than non tolerant M. guttatus after growth in 1 ..mu..M Cu suggesting involvement in the mechanism of tolerance. 22 refs., 2 figs., 1 tab.
- Research Organization:
- Los Alamos National Lab., NM (USA); Liverpool Univ. (UK)
- DOE Contract Number:
- W-7405-ENG-36
- OSTI ID:
- 5520836
- Report Number(s):
- LA-UR-85-2175; CONF-850943-2; ON: DE85014094
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHROMATOGRAPHY
DATA
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
EXPERIMENTAL DATA
FRACTIONATION
INFORMATION
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIQUID COLUMN CHROMATOGRAPHY
METABOLIC ACTIVATION
METALLOPROTEINS
NUCLEI
NUMERICAL DATA
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
SEPARATION PROCESSES
SULFUR 35
SULFUR ISOTOPES
TRACER TECHNIQUES
59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHROMATOGRAPHY
DATA
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
EXPERIMENTAL DATA
FRACTIONATION
INFORMATION
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIQUID COLUMN CHROMATOGRAPHY
METABOLIC ACTIVATION
METALLOPROTEINS
NUCLEI
NUMERICAL DATA
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
SEPARATION PROCESSES
SULFUR 35
SULFUR ISOTOPES
TRACER TECHNIQUES