Identification of tris(. gamma. glutamylcysteinyl)glycines in copper-tolerant mimulus guttatus roots

Conference · Sat Aug 01 04:00:00 EDT 1987 · Plant Physiol., Suppl.; (United States)

OSTI ID:6021369

Robinson, N; Anderson, P; Jackson, P

Copper-tolerant and non-tolerant M. guttatus plants synthesize a cysteine rich copper-complex following exposure to copper. Differences in the amount of this compound in the roots of tolerant and non-tolerant plants indicate that it may be involved in the mechanism of copper tolerance. Partial characterization suggested that this complex was metallothionein (MT)-like, but the structure was not fully elucidated. Poly(..gamma..glutamylcysteinyl)glycines, (..gamma..EC)/sub n/G, have been isolated and characterized from cadmium resistant Datura innoxia cells. These compounds bind metal ions, their synthesis is induced by metals and they appear to act as functional analogues of metallothionein. (..gamma..EC)/sub n/G from cadmium resistant D. innoxia cells was used to calibrate reverse phase HPLC columns (Hi-Pore RP-318, BioRad). Extracts prepared from the roots of M. guttatus plants, grown in the presence of (/sup 35/S) cysteine, were passed through Centricon 30 (Amicon Corp.) columns and then analyzed by reverse phase HPLC. (..gamma..EC)/sub 3/G was detected in extracts from plants grown in the presence of 10 ..mu..M copper for 48 h, but was not apparent in extracts from plants grown in the absence of this metal. It is not known whether the previously isolated putative MT is synonymous with (..gamma..EC)/sub 3/G.

• Research Organization: Los Alamos National Lab., NM
• OSTI ID: 6021369
• Report Number(s): CONF-8707108-
• Conference Information: Journal Name: Plant Physiol., Suppl.; (United States) Journal Volume: 83:4
• Country of Publication: United States
• Language: English

Related Subjects

551000* -- Physiological Systems
59 BASIC BIOLOGICAL SCIENCES
AMIDES
AMINO ACIDS
BIOLOGICAL FUNCTIONS
CADMIUM
CARBOXYLIC ACIDS
CHROMATOGRAPHY
COPPER
CYSTEINE
ELEMENTS
FUNCTIONS
GLUTAMINE
GLYCINE
METALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORNAMENTAL PLANTS
PEPTIDES
PLANTS
POLYPEPTIDES
PROTEINS
ROOTS
SEPARATION PROCESSES
THIOLS
TOLERANCE
TRANSITION ELEMENTS