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Hematopoietic regulatory factors produced in long-term murine bone marrow cultures and the effect of in vitro irradiation

Journal Article · · Blood; (United States)
OSTI ID:6202119
; ; ;
The nature of hematopoietic regulatory factors elaborated by the adherent (stromal) cells of long-term murine bone marrow cultures and the effect of in vitro stromal irradiation (XRT) on the production of these factors was investigated. Using an in situ stromal assay it was possible to demonstrate stromal elaboration of at least two colony-stimulating activities, ie, granulocyte/macrophage colony-stimulating activity (G/M-CSA) and megakaryocyte colony-stimulating activity (Meg-CSA). Exposure of the stroma to XRT resulted in dose-dependent elevations of both activities that correlated inversely with total myeloid cell mass. Mixture experiments that combined control and irradiated stroma revealed that the hematopoietically active control stroma could block detection of XRT-related G/M-CSA elevations. Antiserum directed against purified L cell colony-stimulating factor (CSF) reduced granulocyte/macrophage colony formation in the target layer but did not effect the increased Meg-CSA. While a radioimmunoassay for L-cell type CSF was unable to detect significant differences in concentrated media from control and irradiated cultures, bioassays of these media revealed XRT-related G/M-CSA elevations. These results indicate that the G/M-CSA elaborated in these cultures is immunologically distinct from the Meg-CSA produced, and although distinct from L cell CSF, the G/M-CSA is crossreactive with the L cell CSF antiserum. Morphologic, histochemical, and factor VII antigen immunofluorescent studies were performed on the stromal cell population responsible for production of these stimulatory activities. In addition to ''fat'' cells, the stromal cells remaining after XRT were composed of two predominant cell populations. These included a major population of acid phosphatase and nonspecific esterase-positive macrophage-like cells and a minor population of factor VII antigen negative epithelioid cells.
Research Organization:
Univ. of Virginia School of Medicine, Charlottesville
OSTI ID:
6202119
Journal Information:
Blood; (United States), Journal Name: Blood; (United States) Vol. 64:2; ISSN BLOOA
Country of Publication:
United States
Language:
English

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Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
BONE MARROW CELLS
CELL CULTURES
COLONY FORMATION
CONNECTIVE TISSUE CELLS
DOSE-RESPONSE RELATIONSHIPS
EMISSION SPECTROSCOPY
EXTERNAL IRRADIATION
FLUORESCENCE SPECTROSCOPY
HEMATOPOIETIC SYSTEM
IN VITRO
IRRADIATION
ISOTOPE APPLICATIONS
MACROPHAGES
PHAGOCYTES
PHYSIOLOGY
RADIATION EFFECTS
RADIOASSAY
RADIOIMMUNOASSAY
SOMATIC CELLS
SPECTROSCOPY
TRACER TECHNIQUES