Suppression of marrow stromal cells and microenvironmental damage following sequential radiation and cyclophosphamide. [Mice]

Journal Article · Wed Jul 01 04:00:00 EDT 1981 · Int. J. Radiat. Oncol., Biol. Phys.; (United States)

DOI:https://doi.org/10.1016/0360-3016(81)90012-2· OSTI ID:6200200

Wathen, L M; Knapp, S A; DeGowin, R L

Persistent defects in marrow stroma may contribute to hemopoietic insufficiency in patients treated with combined modality therapy for malignancy. To assess the bone marrow failure following combined therapy, mice received intraperitoneal administration of four weekly doses of cyclophosphamide, 160 mg/kg (CY) one week after 1500 rad at one, two, four and six months post-irradiation. Leg irradiation alone suppressed the repopulation to about 75% of normal and cyclophosphamide alone suppressed to 80% of normal. To directly assess the damage of sequential 1500 rad LI + CY on the microenvironment, marrow stromal cells were flushed from the femoral marrow and cultured as adherent cell colonies. They were suppressed to less than 30% of normal for three months following combined modality treatment. We conclude that multiple courses of cyclophosphamide starting one week after 1500 rad LI produced persistent damage to the microenvironment reflected by decreased marrow stromal cells and faile fraction of pions.

• Research Organization: Univ. of Iowa, Iowa City
• OSTI ID: 6200200
• Journal Information: Int. J. Radiat. Oncol., Biol. Phys.; (United States), Journal Name: Int. J. Radiat. Oncol., Biol. Phys.; (United States) Vol. 7:7; ISSN IOBPD
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATING AGENTS
ANIMAL CELLS
ANIMALS
BIOLOGICAL RECOVERY
BONE MARROW CELLS
CELL CULTURES
CELL PROLIFERATION
CFU
CONNECTIVE TISSUE CELLS
DRUGS
ELECTROMAGNETIC RADIATION
ENDOXAN
IMMUNOSUPPRESSION
IMMUNOSUPPRESSIVE DRUGS
IONIZING RADIATIONS
MAMMALS
MICE
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY EFFECTS
RECOVERY
RODENTS
SOMATIC CELLS
VERTEBRATES
X RADIATION