Characterization of noncovalent metal-oligonucleotide complexes

Conference · Sat Dec 31 04:00:00 EST 1994

OSTI ID:61959

Light-Wahl, K J; Delinger, S L; Gale, D C; Harms, A C; Smith, R D ^[1]; Jenkins, Y; Barton, J K ^[2]

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1. Pacific Northwest Lab., Richland, WA (United States)
2. California Institute of Technology, Pasadena, CA (United States)

Transition metal complexes are being used as probes to determine the conformational structure of oligonucleotides to recognize specific binding sites, and to probe the capability for electron-transfer reactions in DNA strands. For some of the studies transition metal complexes, such as [Ru(phen){sub 2}dppz]{sup 2+}, are tethered to the 5{prime} end of an oligonucleotide chain for use as a spectroscopic probe for the sequence specific detection of oligonucleotides. In other studies, noncovalent interactions, such as intercalation and hydrophobic groove binding, of the metal complexes with oligonucleotide duplexes are used to probe specific DNA sequence and structure. Recent developments with electrospray ionization mass spectrometry (ESI-MS) for the analysis of a wide variety of biomolecules, including oligonucleotides, and importantly the analysis of noncovalent associations has prompted this union of bioinorganic chemistry and mass spectrometry. Presented here are the preliminary results from the use of ESI-MS for the characterization of transition metal-oligonucleotide systems.

• DOE Contract Number: AC06-76RL01830
• OSTI ID: 61959
• Report Number(s): CONF-9405234--
• Country of Publication: United States
• Language: English

Related Subjects

40 CHEMISTRY
55 BIOLOGY AND MEDICINE
BASIC STUDIES
MASS SPECTROSCOPY
MOLECULAR STRUCTURE
OLIGONUCLEOTIDES
RUTHENIUM COMPLEXES