Electrospray/ion trap mass spectrometry of single-stranded and duplex fragments of DNA
- Oak Ridge National Lab., TN (United States)
A particularly interesting, and so far unique, feature of electrospray is its ability to form gaseous ions of non-covalently bound species of biological relevance that are known to exist as specific complexes under physiological conditions. The authors are specifically interested in the role quadrupole ion trap mass spectrometry might play in the analysis of ions derived from specific complexes formed in the condensed-phase. The ion trap has already been demonstrated as a mass analyzer for covalently bound biomolecule-ions derived from cesium ion bombardment, matrix assisted laser desorption, and electrospray. A number of useful experiments have been demonstrated with these ions including high mass resolution, multiple stages of mass spectrometry and ion/molecule reactions. In addition to its merits as an analytical mass spectrometer, the relatively small size and low cost of the ion trap are attractive features which can make it more widely accessible to researchers than more expensive forms of mass spectrometry. For these reasons, it is of interest to explore the possibility for injecting and storing non-covalently associated biomolecule-ions in a quadrupole ion trap for the purpose of mass analysis and for determining what other types of information might be obtainable. The authors have recently reported the injection, storage, and mass analysis of the myoglobin ions, which contain a non-covalently bound heme group, in a quadrupole ion trap. The authors have also focused attention on duplex DNA molecules as an extension of ion trap studies of nucleic acid constituents. Studies to date have emphasized the electrospray, interface, and ion trapping conditions required to observe these species.
- DOE Contract Number:
- AC05-84OR21400
- OSTI ID:
- 61889
- Report Number(s):
- CONF-9405234--
- Country of Publication:
- United States
- Language:
- English
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