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Impatiens pollen germination and tube growth as a bioassay for toxic substances

Journal Article · · Environ. Health Perspect.; (United States)
DOI:https://doi.org/10.1289/ehp.813795· OSTI ID:6187831

Pollen of Impatiens sultanii Hook F. germinates and forms tubes rapidly at 25/sup 0/C in a simple medium containing 111.0 ppm CaCl/sub 2/, 13.6 ppm KH/sub 2/PO/sub 4/, and 1000 ppm boric acid. Calcium, potassium, and boron are essential for germination and tube growth, but sucrose is not required. Pollen tubes grow with equal rapidity in liquid medium or on a medium solidified with 1% agar. Tube growth rates are linear for 1 hr. When different pollen sources or clonal sources are utilized, no variation in pollen tube growth is observed, and pollen from individual flowers remain viable for 26 hr. Formaldehyde inhibits pollen germination, tube production, and tube lengths at 7.5-10 ppm. With 2,4-dichlorophenol, pollen germination and tube production is inhibited at 0.5-20 ppm, while tube growth is inhibited significantly at 25 ppm. A biphasic inhibition of germination and tube formation occurs with p-cresol with a low level of inhibition occurring at 40-60 ppm and a higher one at 100-125 ppm. Tube lengths were inhibited at 150 ppm p-cresol. Acrylamide and dioctyl phthalate have no measurable effect upon pollen germination and tube growth.

Research Organization:
Univ. of Montana, Missoula
OSTI ID:
6187831
Journal Information:
Environ. Health Perspect.; (United States), Journal Name: Environ. Health Perspect.; (United States) Vol. 37; ISSN EVHPA
Country of Publication:
United States
Language:
English

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Related Subjects

560303* -- Chemicals Metabolism & Toxicology-- Plants-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACRYLAMIDE
ALDEHYDES
AMIDES
AROMATICS
BIOASSAY
CHLORINATED AROMATIC HYDROCARBONS
CRESOLS
DATA
ESTERS
EXPERIMENTAL DATA
FORMALDEHYDE
GAMETES
GERM CELLS
GERMINATION
GROWTH
HALOGENATED AROMATIC HYDROCARBONS
HERBS
HYDROXY COMPOUNDS
INFORMATION
NUMERICAL DATA
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHENOLS
PHTHALIC ACID ESTERS
PLANT GROWTH
PLANTS
POLLEN
TOXICITY